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Vinculin Recombinant monoclonal antibody, PBS Only (86930-1-PBS)

Filter: AllWBIHCIF-PIF/ICCAffinity

WB analysis using 86930-1-RR (same clone as 86930-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 86930-1-RR (Vinculin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86930-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 86930-1-RR (same clone as 86930-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86930-1-RR (Vinculin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86930-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 86930-1-RR (same clone as 86930-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86930-1-RR (Vinculin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86930-1-PBS in a different storage buffer formulation.
IF Staining of mouse colon using 86930-1-RR (same clone as 86930-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Vinculin antibody (86930-1-RR, Clone: 251919D10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86930-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Vinculin antibody (86930-1-RR, Clone: 251919D10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86930-1-PBS in a different storage buffer formulation.
IF Staining of mouse colon using 86930-1-RR (same clone as 86930-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Vinculin antibody (86930-1-RR, Clone: 251919D10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86930-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Vinculin antibody (86930-1-RR, Clone: 251919D10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86930-1-PBS in a different storage buffer formulation.
IF Staining of A549 using 86930-1-RR (same clone as 86930-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Vinculin antibody (86930-1-RR, Clone: 251919D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86930-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 86930-1-RR
Biolayer interferometry (BLl) kinetic assays of 86930-1-RR against Human Vinculin were performed. The affinity constant is below 1 pM.

Vinculin Recombinant monoclonal antibody, PBS Only

Name: Vinculin Recombinant monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 86930-1-PBS

Vinculin Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 86930-1-PBS

Clone No.251919D10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

CMD1W, Metavinculin, MV, MVCL, VCL

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

86930-1-PBS targets Vinculin in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag24942 Product name: Recombinant human Vinculin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 777-1066 aa of BC039174 Sequence: PKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ Predict reactive species
Full Name	vinculin
Calculated Molecular Weight	1133 aa, 124 kDa
Observed Molecular Weight	124 kDa
GenBank Accession Number	BC039174
Gene Symbol	Vinculin
Gene ID (NCBI)	7414
RRID	AB_3745213
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P18206
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein that is involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein that is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is a muscle-specific protein.