Vinculin Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86930-1-RR (same clone as 86930-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86930-1-RR (Vinculin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86930-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 86930-1-RR (same clone as 86930-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86930-1-RR (Vinculin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86930-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 86930-1-RR (same clone as 86930-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86930-1-RR (Vinculin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86930-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 86930-1-RR (same clone as 86930-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Vinculin antibody (86930-1-RR, Clone: 251919D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86930-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86930-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86930-1-RR against Human Vinculin were performed. The affinity constant is below 1 pM.

Vinculin Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 86930-1-PBS

Clone No.251919D10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

CMD1W, Metavinculin, MV, MVCL, VCL

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Product Information

86930-1-PBS targets Vinculin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag24942

  Product name: Recombinant human Vinculin protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 777-1066 aa of BC039174

  Sequence: PKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

• Full Name: vinculin
• Calculated Molecular Weight: 1133 aa, 124 kDa
• Observed Molecular Weight: 124 kDa
• GenBank Accession Number: BC039174
• Gene Symbol: Vinculin
• Gene ID (NCBI): 7414
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P18206
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein that is involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein that is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is a muscle-specific protein.