WAC Polyclonal antibody

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• WAC Polyclonal antibody (20519-1-AP)

WAC Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 20519-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

bA48B24, BM 016, DESSH, KIAA1844, PRO1741

• 

  WB analysis using 20519-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20519-1-AP (WAC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 20519-1-AP

  IP result of anti-WAC (IP:20519-1-AP, 4ug; Detection:20519-1-AP 1:600) with HeLa cells lysate 1400 ug.

• 

  IF Staining of MCF-7 using 20519-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using WAC antibody (20519-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20519-1-AP targets WAC in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14355

  Product name: Recombinant human WAC protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 307-649 aa of BC004258

  Sequence: ESTSGDKPVSHSCTTPSTSSASGLNPTSAPPTSASAVPVSPVPQSPIPPLLQDPNLLRQLLPALQATLQLNNSNVDISKINEVLTAAVTQASLQSIIHKFLTAGPSAFNITSLISQAAQLSTQAQPSNQSPMSLTSDASSPRSYVSPRISTPQTNTVPIKPLISTPPVSSQPKVSTPVVKQGPVSQSATQQPVTADKQQGHEPVSPRSLQRSSSQRSPSPGPNHTSNSSNASNATVVPQNSSARSTCSLTPALAAHFSENLIKHVQGWPADHAEKQASRLREEAHNMGTIHMSEICTELKNLRSLVRVCEIQATLREQRILFLRQQIKELEKLKSEFLHGVKM

• Full Name: WW domain containing adaptor with coiled-coil
• Calculated Molecular Weight: 647 aa, 65 kDa
• Observed Molecular Weight: 75-90 kDa
• GenBank Accession Number: BC004258
• Gene Symbol: WAC
• Gene ID (NCBI): 51322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BTA9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WAC is an adaptor protein involved in gene transcription, protein ubiquitination, and autophagy. WAC links the RNA polymerase II transcriptional machinery with the histone H2B monoubiquitination machinery at lysine 120 (H2BK120ub1). It achieves this by using its WW domain to interact with RNA polymerase II and its coiled-coil domain to interact with the RNF20-RNF40 complex, thus coordinating transcriptional output. (PMID: 37402055, PMID: 40653822)