Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells |
| Positive IHC detected in | mouse skeletal muscle tissue, human ovary cancer tissue, mouse testis tissue, rat adrenal gland tissue, mouse adrenal gland tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16370-1-AP targets WAPL in WB, IHC, IF/ICC, ChIP, IP-MS, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9280 Product name: Recombinant human WAPL; WAPAL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-402 aa of BC017393 Sequence: MDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVSSAKALQHCEELIQQYNRAEDSICLADSKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQDGLIGTALNCVLQVPKYLPQEQRFDIRVLLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGTEEKHKKEEEDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC Predict reactive species |
| Full Name | wings apart-like homolog (Drosophila) |
| Calculated Molecular Weight | 142 kDa |
| Observed Molecular Weight | 160-180 kDa |
| GenBank Accession Number | BC017393 |
| Gene Symbol | WAPL |
| Gene ID (NCBI) | 23063 |
| RRID | AB_2216716 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z5K2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The wings apart-like (WAPL, or WAPAL) protein was originally identified as a gene product that potentially regulates heterochromatin organization in Drosophila melanogaster. Human WAPL is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase. Overexpression of WAPL disturbs mitosis and cytokinesis, and contributes to tumor progression by induction of chromosomal instability. WAPAL has a predicted molecular weight of 140 kDa, however, as a result of its extensive phosphorylation it separates on a SDS gel as a band of approximately 160-180 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for WAPL antibody 16370-1-AP | Download protocol |
| IHC protocol for WAPL antibody 16370-1-AP | Download protocol |
| WB protocol for WAPL antibody 16370-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The WAPL Polyclonal antibody (catalog number 16370-1-AP) has 22 citations. These appear in journals including Nature, Nature Immunology, Nature Genetics, Nature Communications, Molecular Cell, Nucleic Acids Research, Journal of Clinical Investigation, Science Advances, Nature Structural & Molecular Biology, Genome Biology, EMBO Journal, Cell Reports, Current Biology, Reproductive Biology and Endocrinology, Frontiers in Cell and Developmental Biology, iScience, BMC Oral Health, and bioRxiv. The research fields span chromatin organization, cohesin/loop extrusion, DNA replication, gene regulation, meiosis, and T cell biology.
| Species | Application | Title |
|---|---|---|
Nature Mechanism for local attenuation of DNA replication at double-strand breaks
| ||
Nat Immunol BRWD1 orchestrates small pre-B cell chromatin topology by converting static to dynamic cohesin | ||
Nat Genet Acute NIPBL depletion reveals in vivo dynamics of loop extrusion and its role in transcription activation. | ||
Nat Genet WAPL maintains a cohesin loading cycle to preserve cell-type-specific distal gene regulation.
| ||
Nat Commun Context-dependent perturbations in chromatin folding and the transcriptome by cohesin and related factors | ||
Nat Commun Interplay between CTCF-binding and CTCF-lacking regulatory elements in generating an architectural stripe at the Igh locus |
Reviews
What customers say
The single review for this antibody reports excellent performance in immunohistochemistry on formalin-fixed, paraffin-embedded mouse testis tissue. The reviewer describes it as very specific and highly effective for IHC staining, using a 1:200 dilution. The product received a 5-star rating, reflecting strong satisfaction with its specificity and staining results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Zhenmin (Verified Customer) (12-05-2019) | This antibody is very specific and works very well with immunohistochemical staining for formalin fixed and paraffin embedded tissue.
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