WAPL Polyclonal antibody

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  WB analysis using 16370-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16370-1-AP (WAPL; WAPAL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 16370-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16370-1-AP (WAPL; WAPAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 16370-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16370-1-AP (WAPL; WAPAL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16370-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WAPL; WAPAL antibody (16370-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

WAPL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16370-1-AP

Publications(13)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Synonyms

WAPL/FOE, FOE, Wings apart-like protein homolog, WAPL cohesin release factor, WAPAL

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IF: See 2 publications below
• ChIP: See 3 publications below

Product Information

16370-1-AP targets WAPL in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9280

  Product name: Recombinant human WAPL; WAPAL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-402 aa of BC017393

  Sequence: MDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVSSAKALQHCEELIQQYNRAEDSICLADSKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQDGLIGTALNCVLQVPKYLPQEQRFDIRVLLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGTEEKHKKEEEDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

• Full Name: wings apart-like homolog (Drosophila)
• Calculated Molecular Weight: 142 kDa
• Observed Molecular Weight: 160-180 kDa
• GenBank Accession Number: BC017393
• Gene Symbol: WAPL
• Gene ID (NCBI): 23063
• RRID: AB_2216716
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7Z5K2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The wings apart-like (WAPL, or WAPAL) protein was originally identified as a gene product that potentially regulates heterochromatin organization in Drosophila melanogaster. Human WAPL is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase. Overexpression of WAPL disturbs mitosis and cytokinesis, and contributes to tumor progression by induction of chromosomal instability. WAPAL has a predicted molecular weight of 140 kDa, however, as a result of its extensive phosphorylation it separates on a SDS gel as a band of approximately 160-180 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB,ChIP	Nat Genet; WAPL maintains a cohesin loading cycle to preserve cell-type-specific distal gene regulation.; Authors - Ning Qing Liu ; KO Validated
human	WB	Sci Adv; RNA polymerase II is required for spatial chromatin reorganization following exit from mitosis.; Authors - Shu Zhang
mouse	WB	J Clin Invest; Allogeneic T cell responses are regulated by a specific miRNA-mRNA network.; Authors - Sun Yaping Y
mouse	WB	Genome Biol; CTCF and cohesin promote focal detachment of DNA from the nuclear lamina; Authors - Tom van Schaik
human	WB	Curr Biol; Esco1 Acetylates Cohesin via a Mechanism Different from That of Esco2.; Authors - Masashi Minamino
mouse	WB,IF	Cell Rep; Cohesin Removal along the Chromosome Arms during the First Meiotic Division Depends on a NEK1-PP1γ-WAPL Axis in the Mouse.; Authors - Miguel A Brieño-Enríquez

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zhenmin (Verified Customer) (12-05-2019)

This antibody is very specific and works very well with immunohistochemical staining for formalin fixed and paraffin embedded tissue.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse Testis

WAPL Antibody Immunohistochemistry, validation (1:200 dilution) in Mouse Testis (Cat no:16370-1-AP)