WARS2 Polyclonal antibody

WARS2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13944-1-AP

Synonyms

Tryptophanyl-tRNA synthetase, Tryptophan--tRNA ligase, mitochondrial, Tryptophan tRNA ligase, TrpRS, EC:6.1.1.2

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Validation Data Gallery

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  WB analysis of human liver using 13944-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13944-1-AP (WARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13944-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13944-1-AP (WARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 13944-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13944-1-AP (WARS2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human liver tissue, mouse brain tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

13944-1-AP targets WARS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WARS2 fusion protein Ag5024
• Full Name: tryptophanyl tRNA synthetase 2, mitochondrial
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 60 kDa/55 kDa
• GenBank Accession Number: BC044575
• Gene Symbol: WARS2
• Gene ID (NCBI): 10352
• RRID: AB_2304541
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WARS2(tryptophan--tRNA ligase, mitochondrial) is also called mitochondrial TRPRS and belongs to the class-I aminoacyl-tRNA synthetase family. The gene encodes the protein containing 360-amino acid ,which has an N-terminal mitochondrial targeting signal that is cleaved after residue 18. WARS2 has characteristics of a class I aminoacyl-tRNA synthetase, including a classical Rossmann fold(PMID:15779907). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse,pig	WB	Virol Sin; Pseudorabies virus manipulates mitochondrial tryptophanyl-tRNA synthetase 2 for viral replication; Authors - Xiu-Qing Li ; KD Validated