WASL Polyclonal antibody

WASL Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14306-1-AP

Synonyms

N WASP, NWASP, WASL, Wiskott Aldrich syndrome like



Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14306-1-AP targets WASL in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WASL fusion protein Ag5424
Full Name Wiskott-Aldrich syndrome-like
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC052955
Gene symbol WASL
Gene ID (NCBI) 8976
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for WASL antibody 14306-1-APDownload protocol
IHC protocol for WASL antibody 14306-1-APDownload protocol
IF protocol for WASL antibody 14306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Mol Cell

The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.

Authors - Joanna R Kovalski
humanWB

Cell Microbiol

The Legionella pneumophila effector WipA disrupts host F-actin polymerisation by hijacking phosphotyrosine signalling.

Authors - Lei He
humanWB

J Dermatol Sci

Negative pressure accelerated monolayer keratinocyte healing involves Cdc42 mediated cell podia formation.

Authors - Hsu Chih-Chin CC
humanWB

Front Immunol

FLI1 Induces Megakaryopoiesis Gene Expression Through WAS/WIP-Dependent and Independent Mechanisms; Implications for Wiskott-Aldrich Syndrome.

Authors - Chunlin Wang