WASL Polyclonal antibody

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  WB analysis of mouse brain using 14306-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14306-1-AP (WASL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14306-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14306-1-AP (WASL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 14306-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14306-1-AP (WASL antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 14306-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14306-1-AP (WASL antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 14306-1-AP

  Immunofluorescent analysis of HepG2 cells, using WASL antibody 14306-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

WASL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14306-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

Actin nucleation-promoting factor WASL, N WASP, Neural Wiskott-Aldrich syndrome protein, NWASP, N-WASP

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

14306-1-AP targets WASL in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5424

  Product name: Recombinant human WASL protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-350 aa of BC052955

  Sequence: MSSVQQQPPPPRRVTNVGSLLLTPQENESLFTFLGKKCVTMSSAVVQLYAADRNCMWSKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDPPNGPNLPMATVDIKNPEITTNRFYGPQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPPPPSRGGPPPPPPPPHNSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPSVAVPPPPPNRMYPPPPPAL

• Full Name: Wiskott-Aldrich syndrome-like
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC052955
• Gene Symbol: WASL
• Gene ID (NCBI): 8976
• RRID: AB_10638478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00401
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Front Immunol; FLI1 Induces Megakaryopoiesis Gene Expression Through WAS/WIP-Dependent and Independent Mechanisms; Implications for Wiskott-Aldrich Syndrome.; Authors - Chunlin Wang
mouse,human	WB,IF,CoIP	iScience; WASH interacts with Ku to regulate DNA double-stranded break repair.; Authors - Tao Wang
human	WB	J Dermatol Sci; Negative pressure accelerated monolayer keratinocyte healing involves Cdc42 mediated cell podia formation.; Authors - Hsu Chih-Chin CC
human	WB	Neuroreport; Decreased expression of cortactin in the schizophrenia brain.; Authors - Hriday P Bhambhvani
human	WB,IHC,IF	Noncoding RNA Res; ADAR1-regulated miR-142-3p/RIG-I axis suppresses antitumor immunity in nasopharyngeal carcinoma; Authors - Haoyuan Xu
human	IF	Mol Cell; The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.; Authors - Joanna R Kovalski