WBP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

WBP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11042-1-AP

Synonyms

WBP 1, WBP1, WW domain binding protein 1

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Validation Data Gallery

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  WB analysis of mouse brain using 11042-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11042-1-AP (WBP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11042-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11042-1-AP (WBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

11042-1-AP targets WBP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WBP1 fusion protein Ag1478
• Full Name: WW domain binding protein 1
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC010012
• Gene Symbol: WBP1
• Gene ID (NCBI): 23559
• RRID: AB_2092061
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WBP1 was originally identified in vitro as a protein that bound to the WW domain of Yes kinase-associated protein. The silencing of WBP1 with small hairpin RNAs caused partial, but significant suppression of ATF6-induced apoptosis. It is a type I transmembrane protein.

Publications

Species	Application	Title
mouse	WB	J Biol Chem; Activating transcription factor-6 (ATF6) mediates apoptosis with reduction of myeloid cell leukemia sequence 1 (Mcl-1) protein via induction of WW domain binding protein 1.; Authors - Morishima Nobuhiro N ; KD Validated