WBP4 Polyclonal antibody

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  WB analysis using 32283-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32283-1-AP (WBP4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

WBP4 Polyclonal Antibody for WB, ELISA

Cat No. 32283-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

FBP21, FNBP21, Formin-binding protein 21, WBP-4, WW domain-binding protein 4

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32283-1-AP targets WBP4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WBP4 fusion protein Ag35400
• Full Name: WW domain binding protein 4 (formin binding protein 21)
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC108310
• Gene Symbol: WBP4
• Gene ID (NCBI): 11193
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O75554
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WW domain-binding protein 4 (WBP4), formerly known as FBP21, is a 376-amino acid spliceosomal protein containing a zinc finger motif and two tandem WW domains. It is a member of the WW domain-containing protein family, which plays a role in various cellular processes including signal transduction, transcriptional regulation and protein degradation. The protein is characterised by its WW domains, which mediate interactions with proline-rich motifs in other proteins. WBP4 is involved in key signalling pathways such as Wnt and Notch and interacts with transcription factors and components of the ubiquitin-proteasome system.