WBSCR17 Polyclonal antibody

WBSCR17 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 21280-1-AP

Synonyms

GalNAc T like protein 3, GALNT16, GALNT20, GALNTL3, pp GaNTase like protein 3, WBSCR17

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Validation Data Gallery

• 

  WB analysis of mouse brain using 21280-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21280-1-AP (WBSCR17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 21280-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21280-1-AP (WBSCR17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21280-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21280-1-AP (WBSCR17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 21280-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21280-1-AP (WBSCR17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 21280-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21280-1-AP (WBSCR17 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 21280-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21280-1-AP (WBSCR17 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 21280-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21280-1-AP (WBSCR17 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 21280-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21280-1-AP (WBSCR17 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, A549 cells, mouse lung tissue, human brain tissue
• Positive IHC detected in: human brain tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21280-1-AP is WBSCR17 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WBSCR17 fusion protein Ag15792
• Full Name: Williams-Beuren syndrome chromosome region 17
• Calculated Molecular Weight: 598 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC069624
• Gene Symbol: WBSCR17
• Gene ID (NCBI): 64409
• RRID: AB_10732815
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.