WDFY1 Polyclonal antibody

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  WB analysis using 13960-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 13960-1-AP (WDFY1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13960-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13960-1-AP (WDFY1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

WDFY1 Polyclonal Antibody for WB, ELISA

Cat No. 13960-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

WD repeat and FYVE domain-containing protein 1, Phosphoinositide-binding protein 1, KIAA1435, FYVE domain-containing protein localized to endosomes 1, FENS-1

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Tested Applications

• Positive WB detected in: THP-1 cells, mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13960-1-AP targets WDFY1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4877

  Product name: Recombinant human WDFY1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-410 aa of BC040525

  Sequence: MAAEIHSRPQSSRPVLLSKIEGHQDAVTAALLIPKEDGVITASEDRTIRVWLKRDSGQYWPSIYHTMASPCSAMAYHHDSRRIFVGQDNGAVMEFHVSEDFNKMNFIKTYPAHQNRVSAIIFSLATEWVISTGHDKCVSWMCTRSGNMLGRHFFTSWASCLQYDFDTQYAFVGDYSGQITLLKLEQNTCSVITTLKGHEGSVACLWWDPIQRLLFSGASDNSIIMWDIGGRKGRTLLLQGHHDKVQSLCYLQLTRQLVSCSSDGGIAVWNMDVSREEAPQWLESDSCQKCEQPFFWNIKQMWDTKTLGLRQHHCRKCGQAVCGKCSSKRSSYPVMGFEFQVRVCDSCYDSIKDEDRTSLATFHEGKHNISHMSMDIARGLMVTCGTDRIVKIWDMTPVVGCSLATGFSPH

• Full Name: WD repeat and FYVE domain containing 1
• Calculated Molecular Weight: 410 aa, 46 kDa
• Observed Molecular Weight: 43-46 kDa
• GenBank Accession Number: BC040525
• Gene Symbol: WDFY1
• Gene ID (NCBI): 57590
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IWB7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WD repeating domain FYVE1 (WDFY1) belongs to the WD40 protein family, which plays key roles in diverse cellular processes, including signal transduction, gene transcription, vesicle fusion, and spermatogenesis(PMID: 32867693; PMID: 10322433). WDFY1 is highly expressed in mouse testis and is dispensable for mouse spermatogenesis and male fertility (PMID: 35121371). The bands of 24 kDa and 19 kDa may be potential isoforms of WDFY1.