WDFY3 Polyclonal antibody

• Featured Product
• KD/KO Validated

WDFY3 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 55009-1-AP

Synonyms

ALFY, Autophagy linked FYVE protein, KIAA0993, WDFY3, ZFYVE25

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Validation Data Gallery

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  IHC staining of rat brain using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 55009-1-AP (WDFY3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55009-1-AP (WDFY3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55009-1-AP (WDFY3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55009-1-AP (WDFY3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55009-1-AP (WDFY3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55009-1-AP (WDFY3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55009-1-AP (WDFY3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55009-1-AP (WDFY3 Antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55009-1-AP (WDFY3 Antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: rat brain tissue, mouse brain tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

55009-1-AP targets WDFY3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: WD repeat and FYVE domain containing 3
• Calculated Molecular Weight: 395 kDa
• GenBank Accession Number: NM_014991
• Gene Symbol: WDFY3
• Gene ID (NCBI): 23001
• RRID: AB_10863126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WDFY3, also named as KIAA0993 and Alfy, is a protein which contains WD repeats and an FYVE domain. Multiple alternatively spliced transcript variants have been found for WDFY3, but the full-length nature of some variants has not been defined. The antibody is specific to WDFY3.The band 50kd in WB is a splicing form of WDFY3.

Publications

Species	Application	Title
mouse	WB,IHC,IF	Cardiovasc Res; WD40 repeat and FYVE domain containing 3 (Wdfy3) is essential for cardiac development.; Authors - Shasha Zhang ; KO Validated
mouse	IHC, IF	In Vitro Cell Dev Biol Anim; Autophagy-linked FYVE protein (Alfy) promotes autophagic removal of misfolded proteins involved in amyotrophic lateral sclerosis (ALS).; Authors - Huihui Han