WDR16 Polyclonal antibody

WDR16 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12644-1-AP

Synonyms

WDRPUH, WD40-repeat protein up-regulated in HCC, WD repeat-containing protein 16, WD repeat domain 16, Cilia- and flagella-associated protein 52

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Validation Data Gallery

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  IHC staining of human testis using 12644-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12644-1-AP (WDR16 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12644-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12644-1-AP (WDR16 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 12644-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12644-1-AP (WDR16 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 12644-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12644-1-AP (WDR16 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 12644-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12644-1-AP (WDR16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human testis tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12644-1-AP targets WDR16 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WDR16 fusion protein Ag3281
• Full Name: WD repeat domain 16
• Calculated Molecular Weight: 620 aa, 68 kDa
• GenBank Accession Number: BC025392
• Gene Symbol: WDR16
• Gene ID (NCBI): 146845
• RRID: AB_2272921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.