WDR18 Polyclonal antibody

• Featured Product
• KD/KO Validated

WDR18 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 15165-1-AP

Synonyms

R32184_1, WD repeat domain 18, WDR18

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Validation Data Gallery

• 

  WB analysis of Jurkat using 15165-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15165-1-AP (WDR18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15165-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15165-1-AP (WDR18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 15165-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15165-1-AP (WDR18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 15165-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15165-1-AP (WDR18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15165-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15165-1-AP (WDR18 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 15165-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15165-1-AP (WDR18 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 15165-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15165-1-AP (WDR18 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary tumor using 15165-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15165-1-AP (WDR18 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human lung tissue, human lung cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IP: See 1 publications below

Product Information

15165-1-AP targets WDR18 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WDR18 fusion protein Ag7282
• Full Name: WD repeat domain 18
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC001648
• Gene Symbol: WDR18
• Gene ID (NCBI): 57418
• RRID: AB_2288469
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WDR18 is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WDR18 may play a role during development. The complex PELP1-TEX10-WDR18 is required for the maturation and nucleolar release of the large ribosomal subunit. This complex is a regulator of ribosome biogenesis and suggests that its SUMO-controlled distribution coordinates the rate of ribosome formation (PMID:21326211).

Publications

Species	Application	Title
human	WB	EMBO J; The SUMO system controls nucleolar partitioning of a novel mammalian ribosome biogenesis complex.; Authors - Finkbeiner Elisabeth E ; KD Validated
human	WB	Mol Cell Proteomics; Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation.; Authors - Fanis Pavlos P
human	WB	PLoS One; A Role of hIPI3 in DNA Replication Licensing in Human Cells.; Authors - Yining Huang
human	WB	Cancer Res Commun; PELP1 is a novel therapeutic target in hepatocellular carcinoma; Authors - Khaled Mohamed Nassar
human	IP,WB	Mol Oncol; PELP1 inhibition by SMIP34 reduces endometrial cancer progression via attenuation of ribosomal biogenesis; Authors - Xue Yang