Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human lung tissue, human lung cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15165-1-AP targets WDR18 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7282 Product name: Recombinant human WDR18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-306 aa of BC001648 Sequence: MAAPMEVAVCTDSAAPMWSCIVWELHSGANLLTYRGGQAGPRGLALLNGEYLLAAQLGKNYISAWELQRKDQLQQKIMCPGPVTCLTASPNGLYVLAGVAESIHLWEVSTGNLLVILSRHYQDVSCLQFTGDSSHFISGGKDCLVLVWSLCSVLQADPSRIPAPRHVWSHHTLPITDLHCGFGGPLARVATSSLDQTVKLWEVSSGELLLSVLFDVSIMAVTMDLAEHHMFCGGSEGSIFQVDLFTWPGQRERSFHPEQDAGKVFKGHRNQVTCLSVSTDGSVLLSGSHDETVRLWDVQSKQCIRT Predict reactive species |
| Full Name | WD repeat domain 18 |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC001648 |
| Gene Symbol | WDR18 |
| Gene ID (NCBI) | 57418 |
| RRID | AB_2288469 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BV38 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
WDR18 is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WDR18 may play a role during development. The complex PELP1-TEX10-WDR18 is required for the maturation and nucleolar release of the large ribosomal subunit. This complex is a regulator of ribosome biogenesis and suggests that its SUMO-controlled distribution coordinates the rate of ribosome formation (PMID:21326211).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for WDR18 antibody 15165-1-AP | Download protocol |
| WB protocol for WDR18 antibody 15165-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The WDR18 Polyclonal antibody (Cat# 15165-1-AP) has 5 citations published in EMBO J, Molecular & Cellular Proteomics, Molecular Oncology, Cancer Research Communications, and PLoS ONE. Research fields span ribosome biogenesis and nucleolar partitioning, chromatin arginine methylation and desumoylation, endometrial cancer progression, hepatocellular carcinoma therapeutics, and DNA replication licensing.
| Species | Application | Title |
|---|---|---|
EMBO J The SUMO system controls nucleolar partitioning of a novel mammalian ribosome biogenesis complex.
| ||
Mol Cell Proteomics Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation. | ||
Mol Oncol PELP1 inhibition by SMIP34 reduces endometrial cancer progression via attenuation of ribosomal biogenesis | ||
Reviews
What customers say
The single review for 15165-1-AP is a 5-star rating from a user who tested the antibody by Western Blot on mouse ES cells at a 1:1000 dilution. The reviewer simply noted "Good for Western Blot," indicating satisfactory performance for that application. Overall, user feedback is positive but limited in volume and detail.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hongwei (Verified Customer) (04-08-2026) | Good for Western Blot
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