WDR19/IFT144 Polyclonal antibody

WDR19/IFT144 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13647-1-AP

Synonyms

IFT144, KIAA1638, ORF26, PWDMP, WD repeat domain 19, WDR19, WDR19/IFT144



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman prostate hyperplasia tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

13647-1-AP targets WDR19/IFT144 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDR19/IFT144 fusion protein Ag4599
Full Name WD repeat domain 19
Calculated Molecular Weight 1342 aa, 152 kDa
Observed Molecular Weight 145-152 kDa
GenBank Accession NumberBC032578
Gene Symbol WDR19
Gene ID (NCBI) 57728
RRIDAB_10598484
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for WDR19/IFT144 antibody 13647-1-APDownload protocol
IHC protocol for WDR19/IFT144 antibody 13647-1-APDownload protocol
IP protocol for WDR19/IFT144 antibody 13647-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Proc Natl Acad Sci U S A

Primary cilia signaling mediates intraocular pressure sensation.

Authors - Na Luo
human

Am J Hum Genet

Patient-iPSC-Derived Kidney Organoids Show Functional Validation of a Ciliopathic Renal Phenotype and Reveal Underlying Pathogenetic Mechanisms.

Authors - Thomas A Forbes
mouseWB

Elife

A WDR35-dependent coat protein complex transports ciliary membrane cargo vesicles to cilia.

Authors - Tooba Quidwai
mouseWB

J Cell Physiol

Ttc39c is upregulated during skeletal muscle atrophy and modulates ERK1/2 MAP kinase and hedgehog signaling.

Authors - Caleb S Hayes
humanWB

PLoS One

Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function.

Authors - Yanan Xu
human

J Assist Reprod Genet

A novel homozygous mutation in WDR19 induces disorganization of microtubules in sperm flagella and nonsyndromic asthenoteratospermia.

Authors - Xiaoqing Ni