Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human prostate hyperplasia tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13647-1-AP targets WDR19/IFT144 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4599 Product name: Recombinant human WDR19 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-353 aa of BC032578 Sequence: MKRIFSLLEKTWLGAPIQFAWQKTSGNYLAVTGADYIVKIFDRHGQKRSEINLPGNCVAMDWDKDGDVLAVIAEKSSCIYLWDANTNKTSQLDNGMRDQMSFLLWSKVGSFLAVGTVKGNLLIYNHQTSRKIPVLGKHTKRITCGCWNAENLLALGGEDKMITVSNQEGDTIRQTQVRSEPSNMQFFLMKMDDRTSAAESMISVVLGKKTLFFLNLNEPDNPADLEFQQDFGNIVCYNWYGDGRIMIGFSCGHFVVISTHTGELGQEIFQARNHKDNLTSIAVSQTLNKVATCGDNCIKIQDLVDLKDMYVILNLDEENKGLGTLSWTDDGQLLALSTQRGSLHVFLTKLPIL Predict reactive species |
| Full Name | WD repeat domain 19 |
| Calculated Molecular Weight | 1342 aa, 152 kDa |
| Observed Molecular Weight | 145-152 kDa |
| GenBank Accession Number | BC032578 |
| Gene Symbol | WDR19 |
| Gene ID (NCBI) | 57728 |
| RRID | AB_10598484 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NEZ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for WDR19/IFT144 antibody 13647-1-AP | Download protocol |
| IP protocol for WDR19/IFT144 antibody 13647-1-AP | Download protocol |
| WB protocol for WDR19/IFT144 antibody 13647-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The WDR19/IFT144 Polyclonal antibody (Cat# 13647-1-AP) has 10 citations spanning journals including Mol Psychiatry, PNAS, Am J Hum Genet, Cell Reports, J Cell Physiology, Mol Biol Cell, PLoS One, J Assist Reprod Genet, Cell Physiol Biochem, and eLife. Research fields cover ciliary biology, intraflagellar transport, cortical and kidney organoid modeling, skeletal muscle atrophy, Hedgehog signaling, and sperm flagellar structure.
| Species | Application | Title |
|---|---|---|
Mol Psychiatry ASD mutations in the ciliary gene CEP41 impact development of projection neurons and interneurons in a human cortical organoid model. | ||
Proc Natl Acad Sci U S A Primary cilia signaling mediates intraocular pressure sensation. | ||
Cell Rep The ciliary gene INPP5E confers dorsal telencephalic identity to human cortical organoids by negatively regulating Sonic hedgehog signaling. | ||
Am J Hum Genet Patient-iPSC-Derived Kidney Organoids Show Functional Validation of a Ciliopathic Renal Phenotype and Reveal Underlying Pathogenetic Mechanisms. | ||
J Cell Physiol Ttc39c is upregulated during skeletal muscle atrophy and modulates ERK1/2 MAP kinase and hedgehog signaling. | ||
Mol Biol Cell Phylogenetic profiling and cellular analyses of ARL16 reveal roles in traffic of IFT140 and INPP5E |
