WDR19/IFT144 Polyclonal antibody

WDR19/IFT144 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13647-1-AP

Synonyms

IFT144, KIAA1638, ORF26, PWDMP, WD repeat domain 19, WDR19



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman prostate hyperplasia tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

13647-1-AP targets WDR19/IFT144 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDR19/IFT144 fusion protein Ag4599
Full Name WD repeat domain 19
Calculated molecular weight 1342 aa, 152 kDa
Observed molecular weight 145-152 kDa
GenBank accession numberBC032578
Gene symbol WDR19
Gene ID (NCBI) 57728
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for WDR19/IFT144 antibody 13647-1-APDownload protocol
IHC protocol for WDR19/IFT144 antibody 13647-1-APDownload protocol
IP protocol for WDR19/IFT144 antibody 13647-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Cell Physiol Biochem

iTRAQ-Based Proteomic Analysis of Neonatal Kidney from Offspring of Protein Restricted Rats Reveals Abnormalities in Intraflagellar Transport Proteins.

Authors - Xiaomei Liu
humanIF

J Assist Reprod Genet

A novel homozygous mutation in WDR19 induces disorganization of microtubules in sperm flagella and nonsyndromic asthenoteratospermia.

Authors - Xiaoqing Ni
humanWB

PLoS One

Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function.

Authors - Yanan Xu
mouseWB

J Cell Physiol

Ttc39c is upregulated during skeletal muscle atrophy and modulates ERK1/2 MAP kinase and hedgehog signaling.

Authors - Caleb S Hayes
humanIF

Am J Hum Genet

Patient-iPSC-Derived Kidney Organoids Show Functional Validation of a Ciliopathic Renal Phenotype and Reveal Underlying Pathogenetic Mechanisms.

Authors - Thomas A Forbes
mouseIF

Proc Natl Acad Sci U S A

Primary cilia signaling mediates intraocular pressure sensation.

Authors - Na Luo