WDR21C Polyclonal antibody

WDR21C Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21571-1-AP

Synonyms

DCAF4L2, WD repeat domain 21C, WDR21C

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Validation Data Gallery

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  WB analysis of HEK-293 using 21571-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21571-1-AP (WDR21C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21571-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21571-1-AP (WDR21C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

The immunogen of 21571-1-AP is WDR21C Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WDR21C fusion protein Ag16098
• Full Name: WD repeat domain 21C
• Calculated Molecular Weight: 395 aa, 44 kDa
• Observed Molecular Weight: 44-50 kDa
• GenBank Accession Number: BC111778
• Gene Symbol: WDR21C
• Gene ID (NCBI): 138009
• RRID: AB_10858783
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Cell Prolif; An approach using Caenorhabditis elegans screening novel targets to suppress tumour cell proliferation.; Authors - Yu-Qin Mao
human	WB,IHC	Am J Transl Res; DCAF4L2 promotes colorectal cancer invasion and metastasis via mediating degradation of NFκb negative regulator PPM1B.; Authors - Haiyu Wang