WDR46 Polyclonal antibody

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• WDR46 Polyclonal antibody (15110-1-AP)

• KD/KO Validated

WDR46 Polyclonal Antibody for WB, FC (Intra), IP, ELISA

Cat No. 15110-1-AP Copied!

Publications (5)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC (Intra), IP, ELISA

Synonyms

BING4, C6orf11, FP221, WD repeat domain 46, WD repeat-containing protein 46

• 

  WB analysis of Jurkat using 15110-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15110-1-AP (WDR46 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 15110-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15110-1-AP (WDR46 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 15110-1-AP

  WB result of WDR46 antibody (15110-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WDR46 transfected HepG2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 15110-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15110-1-AP (WDR46 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of Jurkat using 15110-1-AP

  IP result of anti-WDR46 (IP:15110-1-AP, 4ug; Detection:15110-1-AP 1:8000) with Jurkat cells lysate 2280 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of Jurkat using 15110-1-AP

  IP result of anti-WDR46 (IP:15110-1-AP, 4ug; Detection:15110-1-AP 1:4000) with Jurkat cells lysate 1400 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of Jurkat using 15110-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug WDR46 Polyclonal antibody (15110-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, HepG2 cells, mouse spleen tissue, rat spleen tissue
• Positive IP detected in: Jurkat cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15110-1-AP targets WDR46 in WB, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7095

  Product name: Recombinant human WDR46 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-353 aa of BC000388

  Sequence: METAPKPGKDVPPKKDKLQTKRKKPRRYWEEETVPTTAGASPGPPRNKKNRELRPQRPKNAYILKKSRISKKPQVPKKPREWKNPESQRGLSGAQDPFPGPAPVPVEVVQKFCRIDKSRKLPHSKAKTRSRLEVAEAEEEETSIKAARSELLLAEEPGFLEGEDGEDTAKICQADIVEAVDIASAAKHFDLNLRQFGPYRLNYSRTGRHLAFGGRRGHVAALDWVTKKLMCEINVMEAVRDIRFLHSEALLAVAQNRWLHIYDNQGIELHCIRRCDRVTRLEFLPFHFLLATASETGFLTYLDVSVGKIVAALNARAGRLDVMSQNPYNAVIHLGHSNGTVSLWSPAMKEPLA

• Full Name: WD repeat domain 46
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC000388
• Gene Symbol: WDR46
• Gene ID (NCBI): 9277
• RRID: AB_2215408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15213
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The WDR46 Polyclonal antibody (Cat# 15110-1-AP) has 5 citations published in Genes Dev, Hepatol Commun, Biochem Biophys Res Commun, Biochem Cell Biol, and Genes Cells. Research fields span pluripotent stem cell biology, hepatocellular carcinoma progression, intracellular signaling, SSU processome dynamics, and nucleolar compartmental organization. Applications include Western blot and immunofluorescence in human and mouse samples.

Automatically generated from the 5 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Genes Dev; Role of the small subunit processome in the maintenance of pluripotent stem cells.; Authors - Kwon Tae You
		Hepatol Commun; HBV core protein enhances WDR46 stabilization to upregulate NUSAP1 and promote HCC progression; Authors - Fanyun Kong
mouse	IF	Biochem Biophys Res Commun; Nuclear localization of DMP1 proteins suggests a role in intracellular signaling.; Authors - Siyam Arwa A
human	IF	Biochem Cell Biol; Dynamics of WD-repeat containing proteins in SSU processome components.; Authors - Kouko Wada
human	WB,IF	Genes Cells; Nucleolar scaffold protein, WDR46, determines the granular compartmental localization of nucleolin and DDX21.; Authors - Hirai Yuya Y