WDR46 Polyclonal antibody

WDR46 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15110-1-AP

Synonyms

WDR46, BING4, C6orf11, FP221, WD repeat domain 46, WD repeat-containing protein 46, WD repeat-containing protein BING4

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Validation Data Gallery

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  WB analysis of Jurkat using 15110-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15110-1-AP (WDR46 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis using 15110-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15110-1-AP (WDR46 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15110-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15110-1-AP (WDR46 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15110-1-AP

  IP result of anti-WDR46 (IP:15110-1-AP, 4ug; Detection:15110-1-AP 1:8000) with Jurkat cells lysate 2280 ug.

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  IP experiment of Jurkat using 15110-1-AP

  IP result of anti-WDR46 (IP:15110-1-AP, 4ug; Detection:15110-1-AP 1:4000) with Jurkat cells lysate 1400 ug.

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, mouse spleen tissue, rat spleen tissue
• Positive IP detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 3 publications below

Product Information

15110-1-AP targets WDR46 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WDR46 fusion protein Ag7095
• Full Name: WD repeat domain 46
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC000388
• Gene Symbol: WDR46
• Gene ID (NCBI): 9277
• RRID: AB_2215408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Genes Dev; Role of the small subunit processome in the maintenance of pluripotent stem cells.; Authors - Kwon Tae You
human	IF	Biochem Cell Biol; Dynamics of WD-repeat containing proteins in SSU processome components.; Authors - Kouko Wada
human	WB,IF	Genes Cells; Nucleolar scaffold protein, WDR46, determines the granular compartmental localization of nucleolin and DDX21.; Authors - Hirai Yuya Y
mouse	IF	Biochem Biophys Res Commun; Nuclear localization of DMP1 proteins suggests a role in intracellular signaling.; Authors - Siyam Arwa A