WDR7 Polyclonal antibody

WDR7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24431-1-AP

Synonyms

KIAA0541, Rabconnectin 3 beta, TRAG, WD repeat containing protein 7, WD repeat domain 7, WDR7

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Validation Data Gallery

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  WB analysis using 24431-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24431-1-AP (WDR7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 24431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24431-1-AP (WDR7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 24431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24431-1-AP (WDR7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24431-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24431-1-AP (WDR7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24431-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24431-1-AP (WDR7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human liver tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

The immunogen of 24431-1-AP is WDR7 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WDR7 fusion protein Ag19072
• Full Name: WD repeat domain 7
• Calculated Molecular Weight: 1490 aa, 164 kDa
• Observed Molecular Weight: 145-150 kDa
• GenBank Accession Number: BC131558
• Gene Symbol: WDR7
• Gene ID (NCBI): 23335
• RRID: AB_2879546
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WDR7, also named as KIAA0541 and TRAG, belongs to the WD-repeat protein family. WD repeats are areas with the repetitions of approximately 40 amino acids that have ends with trp-asp and gly-his amino acids (GH-WD) which may help to form a multi-protein complex. WDR7 is the gene encoding adjacent of lincRNA-RoR, which has an important role in apoptosis and cell cycle (PMID: 32128092). WDR7 has 2 isoforms with the molecular mass of 160 and 164 kDa. Sometimes lower molecular weight around 150 kDa can also be observed, which may be a modified variant of WDR7.

Publications

Species	Application	Title
	IF	J Biol Chem; The priming factor CAPS1 regulates dense-core vesicle acidification by interacting with rabconnectin3β/WDR7 in neuroendocrine cells.; Authors - Ellen Crummy