WDR70 Polyclonal antibody

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• WDR70 Polyclonal antibody (33145-1-AP)

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  WB analysis using 33145-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33145-1-AP (WDR70 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

WDR70 Polyclonal Antibody for WB, ELISA

Cat No. 33145-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

WD repeat-containing protein 70

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Tested Applications

• Positive WB detected in: HL-60 cells, MG-63 cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33145-1-AP targets WDR70 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27019

  Product name: Recombinant human WDR70 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 393-649 aa of BC009648

  Sequence: LASRGGDDSLKLWDIRQFNKPLFSASGLPTMFPMTDCCFSPDDKLIVTGTSIQRGCGSGKLVFFERRTFQRVYEIDITDASVVRCLWHPKLNQIMVGTGNGLAKVYYDPNKSQRGAKLCVVKTQRKAKQAETLTQDYIITPHALPMFREPRQRSTRKQLEKDRLDPLKSHKPEPPVAGPGRGGRVGTHGGTLSSYIVKNIALDKTDDSNPREAILRHAKAAEDSPYWVSPAYSKTQPKTMFAQVESDDEEAKNEPEW

• Full Name: WD repeat domain 70
• Calculated Molecular Weight: 654 aa, 73 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC009648
• Gene Symbol: WDR70
• Gene ID (NCBI): 55100
• RRID: AB_3742870
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9NW82
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WD repeat-containing protein 70 (WDR70), which is composed of six WD40 repeat domain functions as a subunit of CRL4 complex in homology-directed repair (HDR) by regulating the transcription of BRCA1, BRCA2, RAD51, and substantial additional transcriptomic changes.