WDR81 Polyclonal antibody

• Featured Product
• KD/KO Validated

WDR81 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24874-1-AP

Synonyms

WD repeat-containing protein 81

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Validation Data Gallery

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  WB analysis using 24874-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24874-1-AP (WDR81 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24874-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24874-1-AP (WDR81 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 24874-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24874-1-AP (WDR81 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 24874-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24874-1-AP (WDR81 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse cerebellum using 24874-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using WDR81 antibody (24874-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 24874-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using WDR81 antibody (24874-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse cerebellum tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

24874-1-AP targets WDR81 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WDR81 fusion protein Ag21666
• Full Name: WD repeat domain 81
• Calculated Molecular Weight: 1941 aa, 212 kDa
• Observed Molecular Weight: 74-80 kDa, 250 kDa
• GenBank Accession Number: BC114568
• Gene Symbol: WDR81
• Gene ID (NCBI): 124997
• RRID: AB_2879771
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WD repeat-containing protein 81 (WDR81) is a 1941 amino acid protein, which contains one BEACH domain and five WD repeats. WDR81 is widely expressed in the brain. WDR81 has an association with cerebellar ataxia, mental retardation, and dysequilibrium syndrome 2. WDR81 exists as six isoforms.

Publications

Species	Application	Title
mouse		Mol Psychiatry; WDR81 regulates adult hippocampal neurogenesis through endosomal SARA-TGFβ signaling.; Authors - Min Wang ; KO Validated
human	WB	Neurogenetics; Fetal brain arrest broadens the spectrum of WDR81-related developmental brain malformations.; Authors - Mohamed S Abdel-Hamid