Tested Applications
| Positive WB detected in | HL-60 cells, Jurkat cells; K-562 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21354-1-AP targets WDR82 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15762 Product name: Recombinant human WDR82 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 175-313 aa of BC018941 Sequence: DLRSFDKGPFATFKMQYDRTCEWTGLKFSNDGKLILISTNGSFIRLIDAFKGVVMHTFGGYANSKAVTLEASFTPDSQFIMIGSEDGKIHVWNGESGIKVAVLDGKHTGPITCLQFNPKFMTFASACSNMAFWLPTIDD Predict reactive species |
| Full Name | WD repeat domain 82 |
| Calculated Molecular Weight | 313 aa, 35 kDa |
| Observed Molecular Weight | 33-35 kDa |
| GenBank Accession Number | BC018941 |
| Gene Symbol | WDR82 |
| Gene ID (NCBI) | 80335 |
| RRID | AB_3085654 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6UXN9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for WDR82 antibody 21354-1-AP | Download protocol |
| WB protocol for WDR82 antibody 21354-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Sci Adv EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency | ||
J Exp Clin Cancer Res Radiotherapy resistance driven by Asparagine endopeptidase through ATR pathway modulation in breast cancer |
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mia (Verified Customer) (07-01-2025) | This antibody was incredibly helpful for my summer project while in medical school. It was used at a 1:100 dilution for staining BHPrS1 cells, and we are planning to drop the dilution to 1:500. It was also used at a 1:200 dilution for IHC of mouse and human prostate tissue, and worked wonderfully.
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