WFS1 Monoclonal antibody

WFS1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4C2B5

Cat no : 67291-1-Ig

Synonyms

FLJ51211, WFRS, WFS, WFS1, Wolfram syndrome 1 (wolframin), WOLFRAMIN



Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells, HEK-293 cells, C6 cells, U-251 cells, PC-12 cells, Neuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67291-1-Ig targets WFS1 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen WFS1 fusion protein Ag25735
Full Name Wolfram syndrome 1 (wolframin)
Calculated Molecular Weight 890 aa, 100 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC030130
Gene Symbol WFS1
Gene ID (NCBI) 7466
RRIDAB_2882556
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss.

Protocols

Product Specific Protocols
WB protocol for WFS1 antibody 67291-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols