NSD3 Polyclonal antibody

• Featured Product
• KD/KO Validated

NSD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, chIP, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 11345-1-AP

Synonyms

WHSC1L1, Protein whistle, pp14328, Nuclear SET domain-containing protein 3, Histone-lysine N-methyltransferase NSD3

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Validation Data Gallery

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  WB analysis of HeLa using 11345-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11345-1-AP (NSD3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11345-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11345-1-AP (NSD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 11345-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11345-1-AP (NSD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11345-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11345-1-AP (NSD3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11345-1-AP

  IP result of anti-NSD3 (IP:11345-1-AP, 4ug; Detection:11345-1-AP 1:600) with HeLa cells lysate 1085 ug.

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  IHC staining of human lymphoma using 11345-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11345-1-AP (NSD3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 11345-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11345-1-AP (NSD3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 11345-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NSD3 antibody (11345-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, rat heart tissue, mouse heart tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 6 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Product Information

11345-1-AP targets NSD3 in WB, IHC, IF/ICC, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NSD3 fusion protein Ag1894
• Full Name: Wolf-Hirschhorn syndrome candidate 1-like 1
• Calculated Molecular Weight: 1437 aa, 162 kDa
• Observed Molecular Weight: 160-170 kDa, 68-72 kDa
• GenBank Accession Number: BC012059
• Gene Symbol: WHSC1L1
• Gene ID (NCBI): 54904
• RRID: AB_2216189
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WHSC1L1, also named as NSD3 and DC28, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is histone methyltransferase. WHSC1L1 is a potently transforming oncogenes based on the number of altered phenotypes expressed by the cells. It contains a PWWP-domain that is a methyl-lysine recognition motif involved in histone code modification and epigenetic regulation of gene expression. Knockdown of WHSC1L1 in 8p11-12-amplified breast cancer cells resulted in profound loss of growth and survival of these cells. (PMID: 20940404). This antibody recognize the long isoform (160-170 kDa) and short isoform (68-72 kDa).

Publications

Species	Application	Title
human	WB	Sci Adv; Arginine methylation of BRD4 by PRMT2/4 governs transcription and DNA repair; Authors - Liu Liu
human	IHC	Cancer Res; NSD3-Induced Methylation of H3K36 Activates NOTCH Signaling to Drive Breast Tumor Initiation and Metastatic Progression.; Authors - Ga-Young Jeong
human	WB	Cancer Res; Transforming properties of 8p11-12 amplified genes in human breast cancer.; Authors - Yang Zeng-Quan ZQ ; KD Validated
		Mol Oncol; Amplification of WHSC1L1 regulates expression and estrogen-independent activation of ERα in SUM-44 breast cancer cells and is associated with ERα over-expression in breast cancer.; Authors - Jonathan C Irish
		Sci Rep; NSD2 contributes to oncogenic RAS-driven transcription in lung cancer cells through long-range epigenetic activation.; Authors - Verónica García-Carpizo
human	WB,IF	Sci Rep; WHSC1L1-mediated EGFR mono-methylation enhances the cytoplasmic and nuclear oncogenic activity of EGFR in head and neck cancer.; Authors - Vassiliki Saloura ; KD Validated

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