WISP3 Polyclonal antibody

WISP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21259-1-AP

Synonyms

CCN family member 6, CCN6, LIBC, PPAC, PPD, WISP 3, WISP3

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Validation Data Gallery

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  WB analysis of Caco-2 using 21259-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 21259-1-AP (WISP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 21259-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21259-1-AP (WISP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21259-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21259-1-AP (WISP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21259-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21259-1-AP (WISP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21259-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21259-1-AP (WISP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Caco-2 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 21259-1-AP is WISP3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WISP3 fusion protein Ag15238
• Full Name: WNT1 inducible signaling pathway protein 3
• Calculated Molecular Weight: 372 aa, 41 kDa
• Observed Molecular Weight: 39-41 kDa
• GenBank Accession Number: BC012028
• Gene Symbol: WISP3
• Gene ID (NCBI): 8838
• RRID: AB_2918070
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Front Mol Biosci; Construction of shared gene signature between rheumatoid arthritis and lung adenocarcinoma helps to predict the prognosis and tumor microenvironment of the LUAD patients; Authors - Liping Shi