WIT1 Polyclonal antibody

WIT1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 15458-1-AP

Synonyms

WIT 1, WIT1

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Validation Data Gallery

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  IHC staining of human nephroblastoma using 15458-1-AP

  Immunohistochemical analysis of paraffin-embedded human nephroblastoma tissue slide using 15458-1-AP (WIT1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human nephroblastoma using 15458-1-AP

  Immunohistochemical analysis of paraffin-embedded human nephroblastoma tissue slide using 15458-1-AP (WIT1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 15458-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15458-1-AP (WIT1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 15458-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15458-1-AP (WIT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 15458-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15458-1-AP (WIT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human ovary tumor tissue, human nephroblastoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15458-1-AP targets WIT1 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WIT1 fusion protein Ag7728
• Full Name: Wilms tumor upstream neighbor 1
• Calculated Molecular Weight: 10 kDa
• GenBank Accession Number: BC002734
• Gene Symbol: WIT1
• Gene ID (NCBI): 51352
• RRID: AB_2878141
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.