WNK4 Polyclonal antibody

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  WB analysis using 22326-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22326-1-AP (WNK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 22326-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22326-1-AP (WNK4 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 22326-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22326-1-AP (WNK4 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 22326-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 22326-1-AP (WNK4 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

WNK4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 22326-1-AP

Publications(3)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Serine/threonine-protein kinase WNK4, Protein kinase lysine-deficient 4, PRKWNK4, PHA2B, EC:2.7.11.1

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Tested Applications

• Positive WB detected in: A375 cells, K-562 cells, PC-12 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

22326-1-AP targets WNK4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WNK4 fusion protein Ag17773
• Full Name: WNK lysine deficient protein kinase 4
• Calculated Molecular Weight: 1243 aa, 135 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC136664
• Gene Symbol: WNK4
• Gene ID (NCBI): 65266
• RRID: AB_2879072
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96J92
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; MST3 Involvement in Na+ and K+ Homeostasis with Increasing Dietary Potassium Intake.; Authors - Chee-Hong Chan
	WB	Biochem J; p62/SQSTM1-mediated selective autophagy is involved in KLHL3-dependent WNK4 degradation.; Authors - Yutaro Mori
mouse	WB	bioRxiv; DOPAMINE D4 RECEPTOR DOWN-REGULATES RENAL SODIUM CHLORIDE COTRANSPORTER VIA UBIQUITINATION-ASSOCIATED LYSOSOME DEGRADATION; Authors - Mingzhuo Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jinwei (Verified Customer) (09-20-2021)

WNK4 Polyclonal antibody (Cat No. 22326-1-AP) works well.

• Applications: Western Blot
• Cell Tissue Type: HEK293

WNK4 Antibody Western Blot validation ( dilution) in HEK293 (Cat no:22326-1-AP)