WNK4 Polyclonal antibody

WNK4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22326-1-AP

Synonyms

PHA2B, PRKWNK4, WNK4



Tested Applications

Positive WB detected inA375 cells, K-562 cells, PC-12 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

22326-1-AP targets WNK4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WNK4 fusion protein Ag17773
Full Name WNK lysine deficient protein kinase 4
Calculated Molecular Weight 1243 aa, 135 kDa
Observed Molecular Weight 180 kDa
GenBank Accession NumberBC136664
Gene Symbol WNK4
Gene ID (NCBI) 65266
RRIDAB_2879072
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for WNK4 antibody 22326-1-APDownload protocol
IHC protocol for WNK4 antibody 22326-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

MST3 Involvement in Na+ and K+ Homeostasis with Increasing Dietary Potassium Intake.

Authors - Chee-Hong Chan
WB

Biochem J

p62/SQSTM1-mediated selective autophagy is involved in KLHL3-dependent WNK4 degradation.

Authors - Yutaro Mori

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jinwei (Verified Customer) (09-20-2021)

WNK4 Polyclonal antibody (Cat No. 22326-1-AP) works well.

  • Applications: Western Blot
  • Cell Tissue Type: HEK293
WNK4 Antibody Western Blot validation ( dilution) in HEK293 (Cat no:22326-1-AP)