Tested Applications
| Positive WB detected in | A375 cells, K-562 cells, PC-12 cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22326-1-AP targets WNK4 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17773 Product name: Recombinant human WNK4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 845-1243 aa of BC136664 Sequence: PISSQVSSNPSPHPTSSPLPFSSSTPEFPVPLSQCPWSSLPTTSPPTFSPTCSQVTLSSPFFPPCPSTSSFPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPSPLPSLPLPPPVAPGGQESPSPHTAEVESEASPPPARPLPGEARLAPISEEGKPQLVGRFQVTSSKEPAEPLPLQPTSPTLSGSPKPSTPQLTSESSDTEDSAGGGPETREALAESDRAAEGLGAGVEEEGDDGKEPQVGGSPQPLSHPSPVWMNYSYSSLCLSSEESESSGEDEEFWAELQSLRQKHLSEVETLQTLQKKEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLSKGSFPTSRRNSLQRSEPPGPGIMRRNSLSGSSTGSQEQRASKGVTFAGDVGRM Predict reactive species |
| Full Name | WNK lysine deficient protein kinase 4 |
| Calculated Molecular Weight | 1243 aa, 135 kDa |
| Observed Molecular Weight | 180 kDa |
| GenBank Accession Number | BC136664 |
| Gene Symbol | WNK4 |
| Gene ID (NCBI) | 65266 |
| RRID | AB_2879072 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96J92 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for WNK4 antibody 22326-1-AP | Download protocol |
| WB protocol for WNK4 antibody 22326-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The WNK4 Polyclonal antibody (Cat# 22326-1-AP) has 8 citations spanning journals including Eur J Pharmacol, Int J Mol Sci, Am J Pathol, Biochem J, Endocrine, Biochem Biophys Res Commun, bioRxiv, and Mol Biol Rep. Research fields covered include ion transport and secretion, Na⁺/K⁺ homeostasis, kidney inflammation, selective autophagy and protein degradation, pseudohypoaldosteronism, asthma, dopamine-receptor-mediated renal transport, and endometrial cancer.
| Species | Application | Title |
|---|---|---|
Eur J Pharmacol Ionic dependency, sensitivity to transport inhibitors and signal transduction of propionate-induced anion secretion in rat caecum. | ||
Int J Mol Sci MST3 Involvement in Na+ and K+ Homeostasis with Increasing Dietary Potassium Intake. | ||
Am J Pathol Low Potassium Triggers Tubular Epithelial NLR Family Pyrin Domain-Containing 3/Apoptosis-Associated Speck-Like Protein-Containing a CARD-Driven Kidney Inflammation Independently of Inflammasomes. | ||
Biochem J p62/SQSTM1-mediated selective autophagy is involved in KLHL3-dependent WNK4 degradation. | ||
Endocrine The functional study of novel KLHL3 missense mutations associated with pseudohypoaldosteronism type II. | ||
Biochem Biophys Res Commun Role of WNK4 and FKBP5 in asthma: Insights from bioinformatics analysis, machine learning, and preliminary validation. |
Reviews
What customers say
One review was submitted for this WNK4 polyclonal antibody used in Western Blot on HEK293 cells. The reviewer rated it 4 out of 5 and reported that the antibody "works well," confirming reliable performance for their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jinwei (Verified Customer) (09-20-2021) | WNK4 Polyclonal antibody (Cat No. 22326-1-AP) works well.
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