WNK4 Polyclonal antibody

WNK4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 22326-1-AP

Synonyms

PHA2B, PRKWNK4, WNK4



Tested Applications

Positive WB detected inmouse colon tissue, mouse liver tissue, mouse lung tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 22326-1-AP is WNK4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WNK4 fusion protein Ag17773
Full Name WNK lysine deficient protein kinase 4
Calculated molecular weight 1243 aa, 135 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC136664
Gene symbol WNK4
Gene ID (NCBI) 65266
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for WNK4 antibody 22326-1-APDownload protocol
IHC protocol for WNK4 antibody 22326-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Biochem J

p62/SQSTM1-mediated selective autophagy is involved in KLHL3-dependent WNK4 degradation.

Authors - Yutaro Mori
mouseWB

Int J Mol Sci

MST3 Involvement in Na+ and K+ Homeostasis with Increasing Dietary Potassium Intake.

Authors - Chee-Hong Chan