WNK4 Polyclonal antibody

WNK4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 22326-1-AP

Synonyms

PHA2B, PRKWNK4, WNK4

---

Validation Data Gallery

• 

  WB analysis using 22326-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22326-1-AP (WNK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 22326-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22326-1-AP (WNK4 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 22326-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22326-1-AP (WNK4 antibody) at dilution of 1:200 (under 40x lens).

---

Tested Applications

• Positive WB detected in: A375 cells, K-562 cells, PC-12 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 22326-1-AP is WNK4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WNK4 fusion protein Ag17773
• Full Name: WNK lysine deficient protein kinase 4
• Calculated molecular weight: 1243 aa, 135 kDa
• Observed molecular weight: 180 kDa
• GenBank accession number: BC136664
• Gene symbol: WNK4
• Gene ID (NCBI): 65266
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; MST3 Involvement in Na+ and K+ Homeostasis with Increasing Dietary Potassium Intake.; Authors - Chee-Hong Chan
	WB	Biochem J; p62/SQSTM1-mediated selective autophagy is involved in KLHL3-dependent WNK4 degradation.; Authors - Yutaro Mori

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Jinwei (Verified Customer) (09-20-2021)

WNK4 Polyclonal antibody (Cat No. 22326-1-AP) works well.

• Applications: Western Blot
• Cell Tissue Type: HEK293

WNK4 Antibody Western Blot validation ( dilution) in HEK293 (Cat no:22326-1-AP)