|Positive WB detected in||A375 cells, K-562 cells, PC-12 cells|
|Positive IHC detected in||human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22326-1-AP is WNK4 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||WNK4 fusion protein Ag17773|
|Full Name||WNK lysine deficient protein kinase 4|
|Calculated molecular weight||1243 aa, 135 kDa|
|Observed molecular weight||180 kDa|
|GenBank accession number||BC136664|
|Gene ID (NCBI)||65266|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jinwei (Verified Customer) (09-20-2021)
WNK4 Polyclonal antibody (Cat No. 22326-1-AP) works well.