WNT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

WNT1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

42

Cat no : 27935-1-AP

Synonyms

Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog, Proto oncogene Wnt 1, Proto oncogene Int 1 homolog, INT1

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Validation Data Gallery

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  WB analysis using 27935-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27935-1-AP (WNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27935-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27935-1-AP (WNT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27935-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27935-1-AP (WNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 27935-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using WNT1 antibody (27935-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 27935-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human WNT1 (27935-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: 3T3-L1 cells, HepG2 cells, NIH/3T3 cells, Jurkat cells
• Positive IF/ICC detected in: NIH/3T3 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 42 publications below
• IHC: See 6 publications below
• IF: See 1 publications below

Product Information

27935-1-AP targets WNT1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WNT1 fusion protein Ag27380
• Full Name: wingless-type MMTV integration site family, member 1
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC074799
• Gene Symbol: WNT1
• Gene ID (NCBI): 7471
• RRID: AB_2881013
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Bioengineered; MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1); Authors - Guang-Lin Song
human	WB	Neural Regen Res; Genetic modification of miR-34a enhances efficacy of transplanted human dental pulp stem cells after ischemic stroke; Authors - Jianfeng Wang
human	WB	Aging (Albany NY); Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway.; Authors - Liansheng Sha
mouse	WB	Microb Biotechnol; Combination of an engineered Lactococcus lactis expressing CXCL12 with light-emitting diode yellow light as a treatment for scalded skin in mice.; Authors - Xiaoxiao Zhao
human	WB,IHC	Front Oncol; Sclerostin Suppression Facilitates Uveal Melanoma Progression Through Activating Wnt/β-Catenin Signaling Via Binding to Membrane Receptors LRP5/LRP6.; Authors - Hanqing Wang
mouse	WB	Transplantation; Agmatine Attenuates Liver Ischemia Reperfusion Injury by Activating Wnt/β-catenin Signaling in Mice.; Authors - Zhenyi Han