WNT10B Polyclonal antibody
WNT10B Polyclonal Antibody for IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 21729-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | NIH/3T3 cells, HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 21729-1-AP is WNT10B Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | WNT10B fusion protein Ag16396 |
Full Name | wingless-type MMTV integration site family, member 10B |
Calculated Molecular Weight | 389 aa, 43 kDa |
GenBank Accession Number | BC096353 |
Gene Symbol | WNT10B |
Gene ID (NCBI) | 7480 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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IHC protocol for WNT10B antibody 21729-1-AP | Download protocol |
IF protocol for WNT10B antibody 21729-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |