WNT10B Polyclonal antibody

WNT10B Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21729-1-AP

Synonyms

Protein Wnt 10b, Protein Wnt 12, SHFM6, WNT 12, WNT10, WNT10B, WNT12



Tested Applications

Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21729-1-AP is WNT10B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WNT10B fusion protein Ag16396
Full Name wingless-type MMTV integration site family, member 10B
Calculated Molecular Weight 389 aa, 43 kDa
GenBank Accession NumberBC096353
Gene Symbol WNT10B
Gene ID (NCBI) 7480
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for WNT10B antibody 21729-1-APDownload protocol
IF protocol for WNT10B antibody 21729-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols