WNT4 Polyclonal antibody

WNT4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14371-1-AP

Synonyms

WNT 4, UNQ426/PRO864, SERKAL, Protein Wnt-4, Protein Wnt 4



Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman kidney tissue, human hepatocirrhosis tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14371-1-AP targets WNT4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WNT4 fusion protein Ag5741
Full Name wingless-type MMTV integration site family, member 4
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39-45 kDa
GenBank Accession NumberBC057781
Gene Symbol WNT4
Gene ID (NCBI) 54361
RRIDAB_2215428
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WNT4 belongs to the Wnt family. WNT4 is critical for embryonic organogenesis, but later plays a unique role among Wnt proteins in the development and maintenance of müllerian and reproductive tissues (PMID: 33963381). The mesenchymal stem cells can improve cardiac function after cardiac injury by regulating endothelial cells via the Wnt4/β-catenin signaling pathway (PMID: 20551912). WNT4 may be modified by glycosylation, leading to an increase in molecular weight.

Protocols

Product Specific Protocols
WB protocol for WNT4 antibody 14371-1-APDownload protocol
IHC protocol for WNT4 antibody 14371-1-APDownload protocol
IF protocol for WNT4 antibody 14371-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Endocrinology

PMID: 31748790

Authors - Alison M Neff
rat

Int J Clin Exp Pathol

Wnt4/β-catenin signaling pathway modulates balloon-injured carotid artery restenosis via disheveled-1.

Authors - Junyi Hua
mouseIHC

Neurochem Res

Wnt Signaling is Altered by Spinal Cord Neuronal Dysfunction in Amyotrophic Lateral Sclerosis Transgenic Mice.

Authors - Yu Li L
humanIF

Cell Death Dis

The transcription factor Sox7 modulates endocardiac cushion formation contributed to atrioventricular septal defect through Wnt4/Bmp2 signaling.

Authors - Nanchao Hong
mouseIHC

BMC Complement Med Ther

Extract of Corallodiscus flabellata attenuates renal fibrosis in SAMP8 mice via the Wnt/β-catenin/RAS signaling pathway.

Authors - Bing Cao
humanWB,IHC

Eur J Pharmacol

HucMSC exosomes attenuate partial bladder outlet obstruction-induced renal injury and cell proliferation via the Wnt/β-catenin pathway

Authors - Zhaoying Wang