WNT6 Polyclonal antibody

WNT6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24201-1-AP

Synonyms

Protein Wnt 6, WNT6



Tested Applications

Positive WB detected inSGC-7901 cells
Positive IHC detected inmouse brain tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24201-1-AP is WNT6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WNT6 fusion protein Ag20889
Full Name wingless-type MMTV integration site family, member 6
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC004329
Gene Symbol WNT6
Gene ID (NCBI) 7475
RRIDAB_2879454
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WNT6 belongs to the Wnt family. It is ligand for members of the frizzled family of seven transmembrane receptors. WNT6 is likely to signal over only few cell diameters. WNT6 may be together with CAV1 to promote chemoresistance of gastric cancer cells to DNA-damaging anthracycline drugs through the activation of the canonical Wnt receptor signaling pathway. And WNT6 is mainly expressed in brain, endometrium epithelium, gastric cancer cell lines and gastric cancer tissues (PMID: 22370641).

Protocols

Product Specific Protocols
WB protocol for WNT6 antibody 24201-1-APDownload protocol
IHC protocol for WNT6 antibody 24201-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,chickenIHC,IF

Development

Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.

Authors - Jianqiong Lin
humanWB

Front Oncol

YTHDF1 Regulates Tumorigenicity and Cancer Stem Cell-Like Activity in Human Colorectal Carcinoma.

Authors - Yang Bai
humanWB

Am J Physiol Cell Physiol

Secreted Wnt6 mediates diabetes-associated centrosome amplification via its receptor FZD4.

Authors - Qin Ju He
humanWB,IHC,IF

Gene

WNT6 participates in the occurrence and development of ovarian cancer by upregulating/activating the typical Wnt pathway and Notch1 signaling pathway

Authors - Haijuan Bao
  • KD Validated
humanWB,IHC

Oncol Lett

Correlation study of DNA methylation of WNT6 gene with osteosarcoma in children.

Authors - Li Li
humanWB

Clin Transl Med

LncRNA NEAT1 suppresses cellular senescence in hepatocellular carcinoma via KIF11-dependent repression of CDKN2A

Authors - Danlei Chen