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  • KD/KO Validated

WRAP53 Polyclonal antibody

WRAP53 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14761-1-AP

Synonyms

TCAB1, WDR79, WRAP53



Tested Applications

Positive WB detected inRaji cells, human kidney tissue, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse testis tissue, human heart tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human spleen tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14761-1-AP targets WRAP53 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WRAP53 fusion protein Ag6522
Full Name WD repeat containing, antisense to TP53
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 72-75 kDa
GenBank Accession NumberBC002336
Gene Symbol WRAP53
Gene ID (NCBI) 55135
RRIDAB_1939806
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for WRAP53 antibody 14761-1-APDownload protocol
IHC protocol for WRAP53 antibody 14761-1-APDownload protocol
IP protocol for WRAP53 antibody 14761-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Genes Dev

The scaffold protein WRAP53β orchestrates the ubiquitin response critical for DNA double-strand break repair.

Authors - Sofia Henriksson
humanWB

Nat Chem Biol

Observation of processive telomerase catalysis using high-resolution optical tweezers.

Authors - Eric M Patrick
humanWB

Oncogenesis

RNF8 ubiquitinates RecQL4 and promotes its dissociation from DNA double strand breaks.

Authors - Qunsong Tan
  • KD Validated
WB

Aging (Albany NY)

Relationship between senescence in macaques and bone marrow mesenchymal stem cells and the molecular mechanism.

Authors - Xing-Hua Pan
humanWB,IF

RNA Biol

Phosphorylation of the Cajal body protein WRAP53β by ATM promotes its involvement in the DNA damage response.

Authors - Christos Coucoravas
  • KD Validated
humanWB

Cancer Cell Int

Suppression of TCAB1 expression induced cellular senescence by lessening proteasomal degradation of p21 in cancer cells.

Authors - Jing Niu