Tested Applications
| Positive WB detected in | Raji cells, human kidney tissue, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse testis tissue, human heart tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human spleen tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 10 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
Product Information
14761-1-AP targets WRAP53 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | WRAP53 fusion protein Ag6522 Predict reactive species |
| Full Name | WD repeat containing, antisense to TP53 |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 72-75 kDa |
| GenBank Accession Number | BC002336 |
| Gene Symbol | WRAP53 |
| Gene ID (NCBI) | 55135 |
| RRID | AB_1939806 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BUR4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
WRAP53 (WD40-encoding RNA Antisense to p53), also known as TCAB1 and WDR79, is a multifunctional protein encoded by a gene that produces both a coding mRNA and a regulatory antisense RNA targeting the tumor suppressor p53. Initially identified for its role in maintaining genomic stability by regulating p53 expression, WRAP53 is crucial for telomerase assembly and localization to Cajal bodies, impacting telomere maintenance. It also participates in DNA damage repair and cellular stress responses. The calculated molecular weight of WRAP53 is about 59 kDa, but the actual observed molecular weight is about 72-75 kDa, which may be due to modification.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WRAP53 antibody 14761-1-AP | Download protocol |
| IHC protocol for WRAP53 antibody 14761-1-AP | Download protocol |
| IP protocol for WRAP53 antibody 14761-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Genes Dev The scaffold protein WRAP53β orchestrates the ubiquitin response critical for DNA double-strand break repair. | ||
Nat Chem Biol Observation of processive telomerase catalysis using high-resolution optical tweezers. | ||
Oncogenesis RNF8 ubiquitinates RecQL4 and promotes its dissociation from DNA double strand breaks.
| ||
Aging (Albany NY) Relationship between senescence in macaques and bone marrow mesenchymal stem cells and the molecular mechanism. | ||
RNA Biol Phosphorylation of the Cajal body protein WRAP53β by ATM promotes its involvement in the DNA damage response.
| ||
Cancer Cell Int Suppression of TCAB1 expression induced cellular senescence by lessening proteasomal degradation of p21 in cancer cells. |
