Multi-rAb® WRAP53 Multi-Recombinant antibody

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• Multi-rAb® WRAP53 Multi-Recombinant antibody (RMX00057)

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  WB analysis using RMX00057

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00057 (WRAP53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using RMX00057

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using RMX00057 (WRAP53 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using RMX00057

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WRAP53 antibody (RMX00057) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

WRAP53 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. RMX00057

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

WDR79, TCAB1, WD repeat-containing protein 79, WD40 repeat-containing protein antisense to TP53 gene, WRAP53beta

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Tested Applications

• Positive WB detected in: HeLa cells, Raji cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00057 targets WRAP53 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: WD repeat containing, antisense to TP53
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 72-75 kDa
• GenBank Accession Number: BC002336
• Gene Symbol: WRAP53
• Gene ID (NCBI): 55135
• RRID: AB_3750108
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: Q9BUR4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WRAP53 (WD40-encoding RNA Antisense to p53), also known as TCAB1 and WDR79, is a multifunctional protein encoded by a gene that produces both a coding mRNA and a regulatory antisense RNA targeting the tumor suppressor p53. Initially identified for its role in maintaining genomic stability by regulating p53 expression, WRAP53 is crucial for telomerase assembly and localization to Cajal bodies, impacting telomere maintenance. It also participates in DNA damage repair and cellular stress responses. The calculated molecular weight of WRAP53 is about 59 kDa, but the actual observed molecular weight is about 72-75 kDa, which may be due to modification.