WRN Polyclonal antibody

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  WB analysis of MCF-7 using 25269-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25269-1-AP (WRN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 25269-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25269-1-AP (WRN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

WRN Polyclonal Antibody for WB, ELISA

Cat No. 25269-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DNA helicase, RecQ like type 3, Exonuclease WRN, RecQ protein like 2, RECQ3, RECQL2, RECQL3, WRN

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Tested Applications

• Positive WB detected in: MCF-7 cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25269-1-AP targets WRN in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: Werner syndrome, RecQ helicase-like
• Calculated Molecular Weight: 162 kDa
• Observed Molecular Weight: 162 kDa
• GenBank Accession Number: NM_000553
• Gene Symbol: WRN
• Gene ID (NCBI): 7486
• RRID: AB_2879997
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14191
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Werner syndrome ATP dependent helicase (WRN) ,also named as RECQ3 or RecQ protein like 2, is a 1432 amino acid protein, which contains one HRDC domain, one helicase C-terminal domain, one helicase ATP-binding domain and 3'-5' exonuclease domain. WRN localizes in the nucleoplasm and belongs to the helicase family. WRN plays a central role in the repair of methylation-induced DNA damage and has an helicase activity necessary to prevent dramatic telomere loss during DNA replication. WRN gene mutation has an association with Werner syndrome and colorectal cancer.