WRN Polyclonal antibody
WRN Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 25269-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 cells, HEK-293 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:200-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
25269-1-AP targets WRN in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | Werner syndrome, RecQ helicase-like |
Calculated Molecular Weight | 162 kDa |
Observed Molecular Weight | 162 kDa |
GenBank Accession Number | NM_000553 |
Gene Symbol | WRN |
Gene ID (NCBI) | 7486 |
RRID | AB_2879997 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Werner syndrome ATP dependent helicase (WRN) ,also named as RECQ3 or RecQ protein like 2, is a 1432 amino acid protein, which contains one HRDC domain, one helicase C-terminal domain, one helicase ATP-binding domain and 3'-5' exonuclease domain. WRN localizes in the nucleoplasm and belongs to the helicase family. WRN plays a central role in the repair of methylation-induced DNA damage and has an helicase activity necessary to prevent dramatic telomere loss during DNA replication. WRN gene mutation has an association with Werner syndrome and colorectal cancer.
Protocols
Product Specific Protocols | |
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WB protocol for WRN antibody 25269-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |