WSTF Polyclonal antibody

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• WSTF Polyclonal antibody (26641-1-AP)

• 

  WB analysis using 26641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26641-1-AP (WSTF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 26641-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using WSTF antibody (26641-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

WSTF Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 26641-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

BAZ1B, EC:2.7.10.2, hWALp2, Tyrosine protein kinase BAZ1B, Tyrosine-protein kinase BAZ1B

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HEK-293 cells, HeLa cells, HepG2 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26641-1-AP targets WSTF in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24403

  Product name: Recombinant human BAZ1B protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1144-1483 aa of BC136520

  Sequence: KVASALEKWKTAIREAQTFSRMHVLLGMLDACIKWDMSAENARCKVCRKKGEDDKLILCDECNKAFHLFCLRPALYEVPDGEWQCPACQPATARRNSRGRNYTEESASEDSEDDESDEEEEEEEEEEEEEDYEVAGLRLRPRKTIRGKHSVIPPAARSGRRPGKKPHSTRRSQPKAPPVDDAEVDELVLQTKRSSRRQSLELQKCEEILHKIVKYRFSWPFREPVTRDEAEDYYDVITHPMDFQTVQNKCSCGSYRSVQEFLTDMKQVFTNAEVYNCRGSHVLSCMVKTEQCLVALLHKHLPGHPYVRRKRKKFPDRLAEDEGDSEPEAVGQSRGRRQKK

• Full Name: bromodomain adjacent to zinc finger domain, 1B
• Calculated Molecular Weight: 1483 aa, 171 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: BC136520
• Gene Symbol: WSTF/BAZ1B
• Gene ID (NCBI): 9031
• RRID: AB_3742196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UIG0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.