WTAP Recombinant antibody, PBS Only

• 

  WB analysis using 81987-4-RR (same clone as 81987-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81987-4-RR (WTAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81987-4-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 81987-4-RR (same clone as 81987-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using WTAP antibody (81987-4-RR, Clone: 250657D4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81987-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 81987-4-RR

  Biolayer interferometry (BLl) kinetic assays of 81987-4-RR against Human WTAP were performed. The affinity constant is 5.11 nM.

WTAP Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 81987-4-PBS

Clone No.250657D4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Female-lethal(2)D homolog (hFL(2)D ), FL2D, KIAA0105, Mum2, Pre-mRNA-splicing regulator WTAP

---

Product Information

81987-4-PBS targets WTAP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: WTAP fusion protein Ag0259
• Full Name: Wilms tumor 1 associated protein
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC000383
• Gene Symbol: WTAP
• Gene ID (NCBI): 9589
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15007
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 45-50 kDa due to phosphorylation.