Willin Polyclonal antibody

Willin Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 21039-1-AP

Synonyms

FRMD6, FERM domain-containing protein 6, FERM domain containing 6, EX1

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Validation Data Gallery

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  WB analysis of U-251 using 21039-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 21039-1-AP (Willin antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 21039-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21039-1-AP (Willin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 21039-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21039-1-AP (Willin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 21039-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21039-1-AP (Willin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 21039-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21039-1-AP (Willin antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of A431 using 21039-1-AP

  1X10^6 A431 cells were stained with .2ug Willin antibody (21039-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: U-251 cells
• Positive IHC detected in: human liver cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21039-1-AP targets Willin in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Willin fusion protein Ag15375
• Full Name: FERM domain containing 6
• Calculated Molecular Weight: 622 aa, 72 kDa
• GenBank Accession Number: BC029870
• Gene Symbol: FRMD6/Willin
• Gene ID (NCBI): 122786
• RRID: AB_2878797
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Willin, also known as FRMD6 or human Expanded (hEx), is a member of the FERM-domain family of cytoskeletal crosslinkers. It is a potent inhibitor of cell proliferation, transformation and modulator of drug sensitivity, suggesting it possesses tumor suppressor properties. Willin is widely expressed in normal tissues. Although willin is found in the cytoplasm and membrane, it localizes to the nuclei of almost all tumors.This antibody was raised against the C-terminal region of human FRMD6 protein and is expected to recognize the endogenous FRMD6.