XAB2 Polyclonal antibody

• Featured Product
• KD/KO Validated

XAB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (2)

Cat no : 10637-1-AP

Synonyms

Pre-mRNA-splicing factor SYF1, PP3898, NTC90, KIAA1177, HCRN

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Validation Data Gallery

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  WB analysis using 10637-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10637-1-AP (XAB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10637-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10637-1-AP (XAB2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10637-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10637-1-AP (XAB2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 10637-1-AP

  IP result of anti-XAB2 (IP:10637-1-AP, 4ug; Detection:10637-1-AP 1:1000) with K-562 cells lysate 1600 ug.

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  IHC staining of mouse salivary gland using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse salivary gland tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse salivary gland using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse salivary gland tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat salivary gland using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat salivary gland using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 10637-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using XAB2 antibody (10637-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells, Y79 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: rat salivary gland tissue, mouse salivary gland tissue, mouse testis tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

10637-1-AP targets XAB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: XAB2 fusion protein Ag0999
• Full Name: XPA binding protein 2
• Calculated Molecular Weight: 100 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC007208
• Gene Symbol: XAB2
• Gene ID (NCBI): 56949
• RRID: AB_2257336
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XPA-binding protein 2 (XAB2) a tetratricopeptide repeat protein involved in transcription-coupled DNA repair and transcription. XAB2 is involved in pre-mRNA splicing, transcription, and transcription-coupled DNA repair, leading to preimplantation lethality, and is essential for mouse embryogenesis. It interactes with Cockayne syndrome groups A and B (CSA and CSB) proteins and RNA polymerase II, which are known to be involved in transcription-coupled repair (TCR) and transcription, and that the antibodies against XAB2 protein inhibited the recovery of RNA synthesis after UV irradiation and normal RNA synthesis when microinjected into living fibroblasts.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.; Authors - Shuai Hou ; KD Validated
human	WB	Nucleic Acids Res; Evidence that a consensus element found in naturally intronless mRNAs promotes mRNA export.; Authors - Lei Haixin H ; KD Validated
human	WB,IF	Cell Death Dis; XAB2 functions in mitotic cell cycle progression via transcriptional regulation of CENPE.; Authors - Shuai Hou ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Moritz (Verified Customer) (08-19-2024)

Antibody worked well for WB, showing a band at ~ 100kDa using HEK293T WT cell lysate, a dilution of 1:3000 and 1h incubation time at RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293T WT lysate

FH

Tsimafei (Verified Customer) (08-12-2024)

Antibody detect a strong band ~ 100 kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESCs

XAB2 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:10637-1-AP)