Tested Applications
Positive WB detected in | Jurkat cells, IFN alpha treated treated Jurkat cells |
Positive IP detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
27057-1-AP targets XAF1 in WB, IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | XAF1 fusion protein Ag25843 Predict reactive species |
Full Name | XIAP associated factor 1 |
Calculated Molecular Weight | 301 aa, 35 kDa |
Observed Molecular Weight | 35 kDa |
GenBank Accession Number | BC073156 |
Gene Symbol | XAF1 |
Gene ID (NCBI) | 54739 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q6GPH4 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
XIAP-associated factor 1 (XAF1) is also named as BIRC4BP (BIRC4-binding protein) and XIAPAF1. XAF1 was originally identified as an antagonist of XIAP counteracting its anti-caspase activity by inhibiting the RING domain of XIAP. XAF1is also concerned with IFN-induced apoptosis (PMID: 35972291). XAF1 plays a key role in the communication between IRF1-mediated early immune responses and IRF3-mediated late IFN-dependent immune responses (PMID: 37595039). XAF1 as a direct interacting protein of AKT1, which strongly binds the N-terminal region of AKT1 to block its K63-linked poly-ubiquitination and subsequent activation (PMID: 37384528). XAF1 may serve as an osteoprotective regulator to restore bone homeostasis (PMID: 38957269).
Protocols
Product Specific Protocols | |
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WB protocol for XAF1 antibody 27057-1-AP | Download protocol |
IP protocol for XAF1 antibody 27057-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |