XAF1 Polyclonal antibody

XAF1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 27057-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

BIRC4 binding protein, BIRC4-binding protein, BIRC4BP, XIAP associated factor 1, XIAPAF1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inJurkat cells, IFN alpha treated treated Jurkat cells
Positive IP detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27057-1-AP targets XAF1 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XAF1 fusion protein Ag25843 Predict reactive species
Full Name XIAP associated factor 1
Calculated Molecular Weight 301 aa, 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC073156
Gene Symbol XAF1
Gene ID (NCBI) 54739
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6GPH4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XIAP-associated factor 1 (XAF1) is also named as BIRC4BP (BIRC4-binding protein) and XIAPAF1. XAF1 was originally identified as an antagonist of XIAP counteracting its anti-caspase activity by inhibiting the RING domain of XIAP. XAF1is also concerned with IFN-induced apoptosis (PMID: 35972291). XAF1 plays a key role in the communication between IRF1-mediated early immune responses and IRF3-mediated late IFN-dependent immune responses (PMID: 37595039). XAF1 as a direct interacting protein of AKT1, which strongly binds the N-terminal region of AKT1 to block its K63-linked poly-ubiquitination and subsequent activation (PMID: 37384528). XAF1 may serve as an osteoprotective regulator to restore bone homeostasis (PMID: 38957269).

Protocols

Product Specific Protocols
WB protocol for XAF1 antibody 27057-1-APDownload protocol
IP protocol for XAF1 antibody 27057-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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