Validation Data Gallery
|Positive WB detected in||HeLa cells, C6 cells, Tunicamycin treated HepG2 cells, HEK-293 cells, NIH/3T3 cells|
|Positive IHC detected in||human colon tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 23453-1-AP is XIAP Fusion Protein expressed in E. coli.
|Tested Reactivity||human, rat, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||XIAP fusion protein Ag0725|
|Full Name||X-linked inhibitor of apoptosis|
|Calculated molecular weight||60 kDa|
|Observed molecular weight||45-50 kDa|
|GenBank accession number||BC032729|
|Gene ID (NCBI)||331|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells. The MW of XIAP is 45-56kd.
Esculetin inhibits endometrial cancer proliferation and promotes apoptosis via hnRNPA1 to downregulate BCLXL and XIAP.
Biochim Biophys Acta Mol Cell Res
HTRA3 transcriptionally inhibited by FOXP1 suppresses tumorigenesis of osteosarcoma via the PTEN/PI3K/AKT pathway
Metabolic Reprogramming Mediates Delayed Apoptosis of Human Neutrophils Infected With Francisella tularensis.
DBDPE upregulates NOD-like receptor signaling to induce NLRP3 inflammasome-mediated HAECs pyroptosis