XIAP Polyclonal antibody

XIAP Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, rat, mouse





Cat no : 23453-1-AP


API3, BIRC4, hIAP 3, hIAP3, hILP, IAP 3, IAP like protein, IAP3, ILP, ILP1, MIHA, X linked IAP, XIAP, XLP2

Tested Applications

Positive WB detected inHeLa cells, C6 cells, Tunicamycin treated HepG2 cells, HEK-293 cells, NIH/3T3 cells
Positive IHC detected inhuman colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23453-1-AP is XIAP Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XIAP fusion protein Ag0725
Full Name X-linked inhibitor of apoptosis
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC032729
Gene Symbol XIAP
Gene ID (NCBI) 331
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells. The MW of XIAP is 45-56kd.


Product Specific Protocols
WB protocol for XIAP antibody 23453-1-APDownload protocol
IHC protocol for XIAP antibody 23453-1-APDownload protocol
IF protocol for XIAP antibody 23453-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cancer Lett

Esculetin inhibits endometrial cancer proliferation and promotes apoptosis via hnRNPA1 to downregulate BCLXL and XIAP.

Authors - Ruqi Jiang

Biochim Biophys Acta Mol Cell Res

HTRA3 transcriptionally inhibited by FOXP1 suppresses tumorigenesis of osteosarcoma via the PTEN/PI3K/AKT pathway

Authors - Ziyuan Tong

Front Immunol

Metabolic Reprogramming Mediates Delayed Apoptosis of Human Neutrophils Infected With Francisella tularensis.

Authors - Samantha J Krysa

Environ Pollut

DBDPE upregulates NOD-like receptor signaling to induce NLRP3 inflammasome-mediated HAECs pyroptosis

Authors - Li Jing