XIRP2 Polyclonal antibody

XIRP2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11896-1-AP

Synonyms

Beta xin, CMYA3, MYOMAXIN, Xeplin, XIRP2

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Validation Data Gallery

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  WB analysis of COLO 320 using 11896-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11896-1-AP (XIRP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 11896-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 11896-1-AP (XIRP2 Antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11896-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11896-1-AP (XIRP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 11896-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 11896-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 11896-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 11896-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse heart using 11896-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using XIRP2 antibody (11896-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: COLO 320 cells, Caco-2 cells, human heart tissue
• Positive IHC detected in: human skeletal muscle tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11896-1-AP targets XIRP2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: XIRP2 fusion protein Ag2515
• Full Name: xin actin-binding repeat containing 2
• Calculated Molecular Weight: 379 kDa
• Observed Molecular Weight: 260-300 kDa
• GenBank Accession Number: BC022888
• Gene Symbol: XIRP2
• Gene ID (NCBI): 129446
• RRID: AB_10951481
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IHC	Elife; Repair of noise-induced damage to stereocilia F-actin cores is facilitated by XIRP2 and its novel mechanosensor domain; Authors - Elizabeth L Wagner
mouse	WB,IF	Front Physiol; Transcriptome Analysis of Cardiac Hypertrophic Growth in MYBPC3-Null Mice Suggests Early Responders in Hypertrophic Remodeling.; Authors - Emily Farrell