XIRP2 Polyclonal antibody

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• XIRP2 Polyclonal antibody (11896-1-AP)

XIRP2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 11896-1-AP Copied!

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

Xin actin-binding repeat-containing protein 2, Xeplin, CMYA3, Cardiomyopathy-associated protein 3, Beta-xin

• 

  WB analysis of COLO 320 using 11896-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11896-1-AP (XIRP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human heart using 11896-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11896-1-AP (XIRP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Caco-2 using 11896-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 11896-1-AP (XIRP2 Antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skeletal muscle using 11896-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skeletal muscle using 11896-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 11896-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 11896-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse heart using 11896-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using XIRP2 antibody (11896-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: COLO 320 cells, Caco-2 cells, human heart tissue
• Positive IHC detected in: human skeletal muscle tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11896-1-AP targets XIRP2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2515

  Product name: Recombinant human XIRP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-199 aa of BC022888

  Sequence: DRRVYAKGETNHNIQQESRTFCKEEFGLTSLGNTSFTDFSCKHPRELREKIPVKQPRICSETRSLSEHFSGMDAFESQIVESKMKTSSSHSSEAGKSGCDFKHAPPTYEDVIAGHILDISDSPKEVRKNFQKTWQESGRVFKGLGYATADASATEMRTTFQEESAFISEAAAPRQGNMYTLSKDSLSNGVPSGRQAEFS

• Full Name: xin actin-binding repeat containing 2
• Calculated Molecular Weight: 379 kDa
• Observed Molecular Weight: 260-300 kDa
• GenBank Accession Number: BC022888
• Gene Symbol: XIRP2
• Gene ID (NCBI): 129446
• RRID: AB_10951481
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A4UGR9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The XIRP2 Polyclonal antibody (catalog number 11896-1-AP) has 4 total citations. These appear in Acta Neuropathologica Communications, Frontiers in Physiology, Biology (Basel), and eLife. The associated research fields span muscle proteomics in titinopathies, cardiac hypertrophic growth and remodeling, XIRP2 mutations in hepatocellular carcinoma, and stereocilia F-actin repair in noise-induced hearing damage. Applications include Western blot, immunofluorescence, and immunohistochemistry across human and mouse samples.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Acta Neuropathol Commun; Phenotype-specific muscle proteomic profiling in titinopathies.; Authors - Aurélien Perrin
mouse	WB,IF	Front Physiol; Transcriptome Analysis of Cardiac Hypertrophic Growth in MYBPC3-Null Mice Suggests Early Responders in Hypertrophic Remodeling.; Authors - Emily Farrell
human	WB,IF	Biology (Basel); Identification of Clinical Value and Biological Effects of XIRP2 Mutation in Hepatocellular Carcinoma; Authors - Dahuan Li ; KD Validated
mouse	WB,IHC	Elife; Repair of noise-induced damage to stereocilia F-actin cores is facilitated by XIRP2 and its novel mechanosensor domain; Authors - Elizabeth L Wagner