XPNPEP1 Polyclonal antibody

XPNPEP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10661-1-AP

Synonyms

Cytosolic aminopeptidase P, SAMP, Soluble aminopeptidase P, X Pro aminopeptidase 1, Xaa Pro aminopeptidase 1, XPNPEP, XPNPEP1, XPNPEPL, XPNPEPL1, XPP1

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Validation Data Gallery

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  WB analysis using 10661-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10661-1-AP (XPNPEP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 10661-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10661-1-AP (XPNPEP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 10661-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10661-1-AP (XPNPEP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 10661-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10661-1-AP (XPNPEP1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human small intestine using 10661-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10661-1-AP (XPNPEP1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas using 10661-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10661-1-AP (XPNPEP1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 10661-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10661-1-AP (XPNPEP1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, mouse small intestine tissue, rat pancreas tissue
• Positive IHC detected in: human small intestine tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10661-1-AP targets XPNPEP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: XPNPEP1 fusion protein Ag1015
• Full Name: X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC007579
• Gene Symbol: XPNPEP1
• Gene ID (NCBI): 7511
• RRID: AB_2215792
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.