XPOT Polyclonal antibody

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  WB analysis using 12577-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12577-1-AP (XPOT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

XPOT Polyclonal Antibody for WB, ELISA

Cat No. 12577-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Exportin T, Exportin(tRNA), Exportin-T, tRNA exportin, XPO3

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, SMMC-7721 cells, Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12577-1-AP targets XPOT in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3256

  Product name: Recombinant human XPOT protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-366 aa of BC020569

  Sequence: MDEQALLGLNPNADSDFRQRALAYFEQLKISPDAWQVCAEALAQRTYSDDHVKFFCFQVLEHQVKYKYSELTTVQQQLIRETLISWLQAQMLNPQPEKTFIRNKAAQVFALLFVTEYLTKWPKFFFDILSVVDLNPRGVDLYLRILMAIDSELVDRDVVHTSEEARRNTLIKDTMREQCIPNLVESWYQILQNYQFTNSEVTCQCLEVVGAYVSWIDLSLIANDRFINMLLGHMSIEVLREEACDCLFEVVNKGMDPVDKMKLVESLCQVLQSAGFFSIDQEEDVDFLARFSKLVNGMGQSLIVSWSKLIKNGDIKNAQEALQAIETKVALMLQLLIHEDDDISSNIIGFCYDYLHILKQLTVLSD

• Full Name: exportin, tRNA (nuclear export receptor for tRNAs)
• Calculated Molecular Weight: 962 aa, 110 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC020569
• Gene Symbol: XPOT
• Gene ID (NCBI): 11260
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43592
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XPOT (also known as Exportin-T) is a key member of the karyopherin-β family of nuclear transport receptors, specifically responsible for the export of a critical class of small non-coding RNAs, transfer RNAs (tRNAs), from the nucleus to the cytoplasm. This process is essential for protein synthesis, as tRNAs must function in the cytoplasm to decode mRNA sequences and deliver amino acids to the growing polypeptide chain. XPOT recognizes mature tRNAs that have been properly processed and aminoacylated in the nucleus, loading them in a RanGTP-dependent manner. Upon translocation through the nuclear pore complex, the hydrolysis of RanGTP to RanGDP in the cytoplasm triggers the release of the tRNA cargo, thereby ensuring that only functional tRNAs are utilized for translation and maintaining the spatial separation of nuclear and cytoplasmic processes.