XPR1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84902-1-RR (same clone as 84902-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84902-1-RR (XPR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84902-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 84902-1-RR (same clone as 84902-1-PBS)

  IP result of anti-XPR1 (IP:84902-1-RR, 4ug; Detection:84902-1-RR 1:1000) with Jurkat cells lysate 1800 ug. This data was developed using the same antibody clone with 84902-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84902-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84902-1-RR against Human XPR1 were performed. The affinity constant is below 1 pM.

XPR1 Uni-rAb^® Recombinant Antibody for WB, IP, Indirect ELISA

Cat No. 84902-1-PBS

Clone No.241788A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Synonyms

241788A7, Phosphate exporter SLC53A1, Protein SYG1 homolog, SLC53A1, Solute carrier family 53 member 1

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Product Information

84902-1-PBS targets XPR1 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5373

  Product name: Recombinant human XPR1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-234 aa of BC041142

  Sequence: MKFAEHLSAHITPEWRKQYIQYEAFKDMLYSAQDQAPSVEVTDEDTVKRYFAKFEEKFFQTCEKELAKINTFYSEKLAEAQRRFATLQNELQSSLDAQKESTGVTTLRQRRKPVFHLSHEERVQHRNIKDLKLAFSEFYLSLILLQNYQNLNFTGFRKILKKHDKILETSRGADWRVAHVEVAPFYTCKKINQLISETEAVVTNELEDGDRQKAMKRLRVPPLGAAQPAPAWTT

• Full Name: xenotropic and polytropic retrovirus receptor
• Calculated Molecular Weight: 696 aa, 82 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC041142
• Gene Symbol: XPR1
• Gene ID (NCBI): 9213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UBH6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

XPR1 is a receptor for xenotropic and polytropic murine leukemia retroviruses and a homolog of yeast Syg1 and plant Pi transporter PHO1 (PMID: 20633538). XPR1 has been identified as an atypical G-protein-coupled receptor. Xenotropic or polytropic retrovirus binding to XPR1 can disrupt the cAMP-mediated signaling function of Xpr1, leading to the apoptosis of infected cells (PMID: 22090134). A band of about 82-100 kDa is probably due to abnormal migration of the protein or post-translation modifications. In addition, a band of about 55-72 kDa may be isoforms and fragments.