XRCC3 Polyclonal antibody

XRCC3 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 18494-1-AP

Synonyms

DNA repair protein XRCC3, XRCC3

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Validation Data Gallery

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  WB analysis of MCF-7 using 18494-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 18494-1-AP (XRCC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 18494-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 18494-1-AP (XRCC3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 18494-1-AP is XRCC3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: XRCC3 fusion protein Ag13317
• Full Name: X-ray repair complementing defective repair in Chinese hamster cells 3
• Calculated molecular weight: 38 kDa
• Observed molecular weight: 38 kDa
• GenBank accession number: BC011725
• Gene symbol: XRCC3
• Gene ID (NCBI): 7517
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Int J Biochem Cell Biol; MiroRNA-127-3p targets XRCC3 to enhance the chemosensitivity of esophageal cancer cells to a novel phenanthroline-dione derivative.; Authors - Ni Zhen
human	WB	Nat Struct Mol Biol; Fanconi anemia DNA crosslink repair factors protect against LINE-1 retrotransposition during mouse development; Authors - Nazareno Bona