XRCC3 Polyclonal antibody

• 

  WB analysis of MCF-7 using 18494-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 18494-1-AP (XRCC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 18494-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 18494-1-AP (XRCC3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

XRCC3 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 18494-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

X-ray repair cross-complementing protein 3, CMM6

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

18494-1-AP targets XRCC3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13317

  Product name: Recombinant human XRCC3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-346 aa of BC011725

  Sequence: MDLDLLDLNPRIIAAIKKAKLKSVKEVLHFSGPDLKRLTNLSSPEVWHLLRTASLHLRGSSILTALQLHQQKERFPTQHQRLSLGCPVLDALLRGGLPLDGITELAGRSSAGKTQLALQLCLAVQFPRQHGGLEAGAVYICTEDAFPHKRLQQLMAQQPRLRTDVPGELLQKLRFGSQIFIEHVADVDTLLECVNKKVPVLLSRGMARLVVIDSVAAPFRCEFDSQASAPRARHLQSLGAMLRELSSAFQSPVLCINQVTEAMEEQGAAHGPLGFWDERVSPALGITWANQLLVRLLADRLREEEAALGCPARTLRVLSAPHLPPSSCSYTISAEGVRGTPGTQSH

• Full Name: X-ray repair complementing defective repair in Chinese hamster cells 3
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC011725
• Gene Symbol: XRCC3
• Gene ID (NCBI): 7517
• RRID: AB_2878546
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43542
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Death Dis; Targeting FBXO22 enhances radiosensitivity in non-small cell lung cancer by inhibiting the FOXM1/Rad51 axis; Authors - Yunshang Chen
human	WB	Int J Biochem Cell Biol; MiroRNA-127-3p targets XRCC3 to enhance the chemosensitivity of esophageal cancer cells to a novel phenanthroline-dione derivative.; Authors - Ni Zhen
human	WB	Nat Struct Mol Biol; Fanconi anemia DNA crosslink repair factors protect against LINE-1 retrotransposition during mouse development; Authors - Nazareno Bona