XRCC3 Monoclonal antibody

XRCC3 Monoclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A12F9

Cat no : 67222-1-Ig

Synonyms

DNA repair protein XRCC3, XRCC3



Tested Applications

Positive WB detected inNCI-H1299 cells, HT-29 cells, Caco-2 cells, HT-1080 cells, HeLa cells, MCF-7 cells, COLO 320 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inmouse testis tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:150-1:600
Sample-dependent, check data in validation data gallery

Product Information

67222-1-Ig targets XRCC3 in WB, IP, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen XRCC3 fusion protein Ag13317
Full Name X-ray repair complementing defective repair in Chinese hamster cells 3
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC011725
Gene symbol XRCC3
Gene ID (NCBI) 7517
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for XRCC3 antibody 67222-1-IgDownload protocol
IHC protocol for XRCC3 antibody 67222-1-IgDownload protocol
IP protocol for XRCC3 antibody 67222-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols