XRCC4 Monoclonal antibody

XRCC4 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

3E5D2

Cat no : 66621-1-Ig

Synonyms

DNA repair protein XRCC4, XRCC4



Tested Applications

Positive WB detected inHEK-293, A431, HepG2 cells, COLO 320 cells, MCF-7 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

66621-1-Ig targets XRCC4 in WB, IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen XRCC4 fusion protein Ag8807
Full Name X-ray repair complementing defective repair in Chinese hamster cells 4
Calculated molecular weight 310 aa, 35 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC005259
Gene symbol XRCC4
Gene ID (NCBI) 7518
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for XRCC4 antibody 66621-1-IgDownload protocol
IHC protocol for XRCC4 antibody 66621-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Immunity

Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation.

Authors - Yan Wang
  • KD Validated