XRCC4 Monoclonal antibody

• Featured Product
• KD/KO Validated

XRCC4 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3E5D2

Cat no : 66621-1-Ig

Synonyms

DNA repair protein XRCC4, XRCC4

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Validation Data Gallery

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  WB analysis of HEK-293 using 66621-1-Ig

  WB result of XRCC4 antibody (66621-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-XRCC4 transfected HEK-293 cells.

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  WB analysis using 66621-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66621-1-Ig (XRCC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 66621-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66621-1-Ig (XRCC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66621-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66621-1-Ig (XRCC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66621-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66621-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66621-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66621-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, COLO 320 cells, MCF-7 cells, A431 cells, HepG2 cells
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

66621-1-Ig targets XRCC4 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: XRCC4 fusion protein Ag8807
• Full Name: X-ray repair complementing defective repair in Chinese hamster cells 4
• Calculated Molecular Weight: 310 aa, 35 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC005259
• Gene Symbol: XRCC4
• Gene ID (NCBI): 7518
• RRID: AB_2881981
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Immunity; Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation.; Authors - Yan Wang ; KD Validated