XYLT1 Polyclonal antibody

XYLT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 55061-1-AP

Synonyms

Peptide O xylosyltransferase 1, XT I, XT1, Xylosyltransferase 1, xylosyltransferase I, XylT I, XYLT1

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Validation Data Gallery

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  WB analysis of HEK-293 using 55061-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55061-1-AP (XYLT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 55061-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 55061-1-AP (XYLT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 55061-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55061-1-AP (XYLT1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 55061-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55061-1-AP (XYLT1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 55061-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 55061-1-AP (XYLT1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse pancreas using 55061-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 55061-1-AP (XYLT1 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, BxPC-3 cells
• Positive IHC detected in: mouse kidney tissue, mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

55061-1-AP targets XYLT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: xylosyltransferase I
• Calculated Molecular Weight: 108 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: NM_022166
• Gene Symbol: XYLT1
• Gene ID (NCBI): 64131
• RRID: AB_10804758
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XYLT1, also named as XT1, Belongs to the glycosyltransferase 14 family and XylT subfamily. It catalyzes the first step in biosynthesis of glycosaminoglycan. XYLT1 transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Initial enzyme in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans in fibroblasts and chondrocytes. The antibody is specific to XYLT1.

Publications

Species	Application	Title
mouse	WB	Phytomedicine; Gua Lou Er Chen decoction attenuates atherosclerosis by reducing proteoglycans accumulation and inflammation; Authors - Hongya Guo
human	WB	J Biol Chem; Chondroitin sulfate modification of CSPG4 regulates the maintenance and differentiation of glioma initiating cells via integrin-associated signaling; Authors - Akiko Niibori-Nambu