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Tested Applications
| Positive IP detected in | MDA-MB-231 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55064-1-AP targets XYLT2 in IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | xylosyltransferase II |
| Calculated Molecular Weight | 97 kDa |
| Observed Molecular Weight | 97-110 kDa |
| GenBank Accession Number | NM_022167 |
| Gene Symbol | XYLT2 |
| Gene ID (NCBI) | 64132 |
| RRID | AB_3743198 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H1B5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Xylosyltransferases catalyze the attachment of xylose, first linker sugar residue, to a major group of extracellular matrix proteins, namely proteoglycans, and are therefore of key importance for proper PG biosynthesis in many tissues (PMID: 26027496). XYLT2 (xylosyltransferase II, XylT-II) is a protein homologous to XYLT1 but with hitherto unknown activity or physiological function. In most tissues, both xylosyltransferase genes are expressed and in numerous cell lines XYLT2 is the major xylosyltransferase (PMID: 17189265, 25748573).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for XYLT2 antibody 55064-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
