YARS2 Polyclonal antibody

YARS2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17150-1-AP

Synonyms

CGI 04, mt TyrRS, Tyrosine tRNA ligase, TYRRS, YARS2



Tested Applications

Positive WB detected inK-562 cells, HepG2 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman testis tissue, human placenta tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17150-1-AP targets YARS2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YARS2 fusion protein Ag9530
Full Name tyrosyl-tRNA synthetase 2, mitochondrial
Calculated Molecular Weight 477 aa, 53 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC015625
Gene Symbol YARS2
Gene ID (NCBI) 51067
RRIDAB_2219263
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for YARS2 antibody 17150-1-APDownload protocol
IHC protocol for YARS2 antibody 17150-1-APDownload protocol
IF protocol for YARS2 antibody 17150-1-APDownload protocol
IP protocol for YARS2 antibody 17150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols