Filter: AllWBIHCIF/ICC

WB analysis using 15473-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 15473-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IHC staining of human endometrial cancer using 15473-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15473-1-AP (YIPF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human endometrial cancer using 15473-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15473-1-AP (YIPF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15473-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using YIPF4 antibody (15473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HeLa using 15473-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15473-1-AP (YIPF4 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Red: CL594-phalloidin staining of F-actin.

YIPF4 Polyclonal antibody

Name: YIPF4 Polyclonal antibody
Brand: Proteintech
SKU: 15473-1-AP

YIPF4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15473-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FinGER4, Nbla11189, Protein YIPF4, Yip1 domain family, member 4, YIP1 family member 4, YIPF4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

Positive WB detected in	HeLa cells, HEK-293T cells, Jurkat cells, HaCaT cells
Positive IHC detected in	human endometrial cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

WB	See 2 publications below

15473-1-AP targets YIPF4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7777 Product name: Recombinant human YIPF4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-113 aa of BC004875 Sequence: MQPPGPPPAYAPTNGDFTFVSSADAEDLSGSIASPDVKLNLGGDFIKESTATTFLRQRGYGWLLEVEDDDPEDNKPLLEELDIDLKDIYYKIRCVLMPMPSLGFNRQVVRDNP Predict reactive species
Full Name	Yip1 domain family, member 4
Calculated Molecular Weight	27 kDa
Observed Molecular Weight	27 kDa
GenBank Accession Number	BC004875
Gene Symbol	YIPF4
Gene ID (NCBI)	84272
RRID	AB_2217206
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BSR8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IF protocol for YIPF4 antibody 15473-1-AP	Download protocol
IHC protocol for YIPF4 antibody 15473-1-AP	Download protocol
WB protocol for YIPF4 antibody 15473-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB	Adv Sci (Weinh) Blocking Lysine Crotonylation and Aerobic Glycolysis as Targeting Strategy Against mpox Virus Replication. Authors - Pengjun Wei View Article
		Dev Cell TM9SF3 is a Golgi-resident ATG8-binding protein essential for Golgi-selective autophagy Authors - Jiejie Yang View Article
human	WB	Nat Struct Mol Biol DMXL1 promotes recruitment of V1-ATPase to lysosomes upon TRPML1 activation Authors - Chan Lee View Article