YOD1 Recombinant antibody, PBS Only

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  WB analysis using 84933-5-RR (same clone as 84933-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84933-5-RR (YOD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84933-5-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 84933-5-RR (same clone as 84933-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using YOD1 antibody (84933-5-RR, Clone: 242498G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84933-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84933-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84933-5-RR against Human YOD1 were performed. The affinity constant is 1.07 nM.

YOD1 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 84933-5-PBS

Clone No.242498G6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

242498G6, DUBA 8, DUBA8, DUBA-8, EC:3.4.19.12

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Product Information

84933-5-PBS targets YOD1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: YOD1 fusion protein Ag21844
• Full Name: YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae)
• Calculated Molecular Weight: 348 aa, 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC137167
• Gene Symbol: YOD1
• Gene ID (NCBI): 55432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q5VVQ6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

YOD1, also known as YOD1 deubiquitinase, belongs to the ovarian tumor (OTU) domain-containing family of deubiquitinating enzymes (DUBs), which play a crucial role in regulating cellular processes by removing ubiquitin molecules from target proteins. YOD1 is particularly involved in the endoplasmic reticulum-associated degradation (ERAD) pathway, where it assists in the clearance of misfolded proteins from the endoplasmic reticulum (ER) by modulating the ubiquitination status of ERAD components. (PMID: 40132762,19818707)