YPEL3 Polyclonal antibody

• Featured Product
• KD/KO Validated

YPEL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15403-1-AP

Synonyms

Protein yippee like 3, yippee like 3 (Drosophila), YPEL3

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Validation Data Gallery

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  WB analysis of BxPC-3 using 15403-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15403-1-AP (YPEL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human endometrial cancer using 15403-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15403-1-AP (YPEL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 15403-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15403-1-AP (YPEL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: BxPC-3 cells
• Positive IHC detected in: human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

15403-1-AP targets YPEL3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YPEL3 fusion protein Ag7626
• Full Name: yippee-like 3 (Drosophila)
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC005009
• Gene Symbol: YPEL3
• Gene ID (NCBI): 83719
• RRID: AB_11042587
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YPEL3 is part of a five-member family of closely related paralogues: YPEL1-5, which are named in reference to their Drosophila ortholgue. Studies showed shat YPEL3 is regulated by p53 and its encoding protein induces cellular senescence in human tumor and normal cells, indicating that YPEL3 is a p53-dependent, tumor suppressor gene. (PMID:20388804) 15403-1-AP was raised against full length of YPEL3 protein (1-119aa); it can recognize YPEL1-4.

Publications

Species	Application	Title
human	WB	Cancer Res; YPEL3, a p53-regulated gene that induces cellular senescence.; Authors - Kelley Kevin D KD ; KD Validated
human	WB	Int J Cancer; Novel senescence associated gene, YPEL3, is repressed by estrogen in ER+ mammary tumor cells and required for tamoxifen-induced cellular senescence.; Authors - Tuttle Rebecca R
human	WB,IF	Sci Rep; Steroid sulfatase deficiency causes cellular senescence and abnormal differentiation by inducing Yippee-like 3 expression in human keratinocytes.; Authors - Hyoung-Seok Baek
human	IHC	Front Genet; Identification of a cellular senescence-related-lncRNA (SRlncRNA) signature to predict the overall survival of glioma patients and the tumor immune microenvironment; Authors - Qing Liu